Molecular Analysis of the VP1/AB13 Activator-Repressor

小行星9724000 这一建议继续我们的调查基础VP 1介导的基因激活和抑制的分子机制。玉米的VP 1因子在发育中的种子的成熟和萌发途径的整合中起核心作用。 VP 1/AB 13家族是植物界特有的调节蛋白。 最近的研究表明，相关蛋白在植物发育的其他阶段发挥作用。 McCarty博士已经表明，VP 1的功能取决于靶启动子作为激活剂或阻遏物，他最近已经能够证明合作序列特异性DNA结合活性。 他将讨论三个关键问题： 1)VP 1的B3 DNA结合域的独特性质和调控。 2)启动子环境被解释以决定VP 1是激活还是抑制转录的机制。 3)鉴定提供背景信息和介导阻遏的伴侣蛋白。

9724000 McCarty This proposal continues our investigation of the molecular mechanisms that underlie VP1 mediated gene activation and repression. The VP1 factor of maize plays a central role in integration of the maturation and germination pathways in the developing seed. The VP1/AB13 family of regulatory proteins is unique to the plant kingdom. Recent studies indicate that related proteins function in other phases of plant development. Dr. McCarty has shown that VP1 functions as an activator or repressor depending upon the target promoter and he has recently been able to demonstrate a cooperative sequence specific DNA binding activity. He will address three key issues: 1) The unique properties and regulation of the B3 DNA binding domain of VP1. 2) The mechanism by which promoter context is interpreted to determine whether VP1 activates or represses transcription. 3) The identification of partner proteins that provide the context information and mediate repression.