Molecular Analysis of the VP1/AB13 Activator-Repressor

这一建议继续我们对VP1介导的基因激活和抑制的分子机制的研究。VP1因子在玉米种子发育过程中整合成熟和萌发途径中起着核心作用。VP1/AB13家族调控蛋白是植物界独有的。最近的研究表明，相关蛋白在植物发育的其他阶段也起作用。McCarty博士已经证明VP1的激活或抑制作用取决于目标启动子，他最近已经能够证明一个合作序列特定的DNA结合活性。他将解决三个关键问题：1)VP1的B3 DNA结合域的独特性质和调控。2)启动子上下文被解释以确定VP1是否激活或抑制转录的机制。3)提供上下文信息和介导抑制的伙伴蛋白的鉴定。

9724000 McCarty This proposal continues our investigation of the molecular mechanisms that underlie VP1 mediated gene activation and repression. The VP1 factor of maize plays a central role in integration of the maturation and germination pathways in the developing seed. The VP1/AB13 family of regulatory proteins is unique to the plant kingdom. Recent studies indicate that related proteins function in other phases of plant development. Dr. McCarty has shown that VP1 functions as an activator or repressor depending upon the target promoter and he has recently been able to demonstrate a cooperative sequence specific DNA binding activity. He will address three key issues: 1) The unique properties and regulation of the B3 DNA binding domain of VP1. 2) The mechanism by which promoter context is interpreted to determine whether VP1 activates or represses transcription. 3) The identification of partner proteins that provide the context information and mediate repression.