Construction of Comprehensive Sequence Indexed Transposon Resources for Maize

PI：唐纳德·麦卡蒂，佛罗里达大学CoPI：卡伦·科赫，佛罗里达大学合作者：罗布·马蒂恩森，W。Richard McCombie，冷泉港植物园合作者：Carolyn Lawrence，爱荷华州州立大学玉米是植物生物学和遗传学研究的重要模式生物，也是世界上经济上最重要的作物之一。 目前正在进行的玉米基因组的完整测序将使研究人员能够确定制造玉米植物所需的全套基因。 为了了解这些基因的功能和相互作用，研究人员需要系统地产生破坏每个基因的基因突变的方法。 为此，已经产生了含有活性Mutator转座子的玉米植物的大群体，以使基因组充满插入突变。 为了使世界各地的不同玉米研究人员能够容易地识别和获得感兴趣的基因中的突变，将为UniformMu近交玉米群体创建基因组中每个插入位点位置的基于DNA序列的索引。 序列索引数据库预计将包含8050个玉米品系集合中多达100，000个独特转座子插入位点的注释。 该项目的更广泛影响将包括为不同的本科生提供生物信息学、最先进的DNA测序技术、玉米遗传学和转座子生物学方面的丰富研究经验。 该资源的公共接口将通过MaizeGDB.org门户网站提供。这将使个别研究人员能够识别感兴趣的突变，并从玉米遗传合作库存中心获得突变体股票的种子。

PI: Donald McCarty, University of FloridaCoPI: Karen Koch, University of FloridaCollaborators: Rob Martienssen, W. Richard McCombie, Cold Spring Harbor LaboratoriesCollaborator: Carolyn Lawrence, Iowa State UniversityMaize is a key model organism for plant biology and genetics research in addition to being among the world''''s economically most important crops. The complete sequencing of the maize genome currently in progress will enable researchers to identify the complete set of genes required to make a maize plant. In order to understand the functions and interactions of those genes, researchers will require methods for systematically generating genetic mutations that disrupt each gene. For this purpose, large populations of maize plants containing active Mutator transposons have been created to saturate the genome with insertional mutations. To enable diverse maize researcher's world-wide to easily identify and obtain mutations in genes of interest, a DNA sequence based index of the location of each insertion site in the genome will be created for the UniformMu inbred maize population. The sequence index database is expected to contain annotations of up to 100,000 unique transposon insertion sites in a collection of 8050 maize lines. The broader impacts of the project will include providing rich research experiences in bioinformatics, state-of-the-art DNA sequencing technologies, maize genetics and transposon biology to diverse undergraduates. A public interface to the resource will be provided through the MaizeGDB.org web portal. These will enable individual researchers to identify mutations of interest and obtain seed of mutant stocks from the Maize Genetics Cooperation Stock Center.