Heterogeneous Kinetics of the Major Thermomonospora fusca and Trichoderma reesei Cellulases

在酶水解过程中，纤维素颗粒受到 纤维素酶导致可发酵糖的产生。 然而，在生产大量的可发酵的 糖，初始纤维素颗粒的相当大的碎片 发生。 尽管几位调查人员对此进行了报道 然而，很少有人努力评估这一比率， 分裂的程度，也没有分析的影响， 纤维素结构特征和纤维素酶特性对纤维素酶活性的影响 碎片化的速度和程度。 本次调查的目的 是用实验和数学方法分析纤维素 碎片化 平均粒径、纤维素孔 体积和结晶度，以及纤维素酶的特性， 对褐热单孢菌的裂解速率和程度的影响 分析了 纤维素是生物圈产生的最丰富的碳水化合物。 它是植物细胞壁的主要组成部分。 此外，纤维素 占全国城市固体废物产生量的40% 全国 在过去的10年里， 将这些可再生资源用于工业化学品的兴趣 和食物。 这种兴趣的一部分是由相对较低的 纤维素材料的成本。 许多微生物产生 酶（纤维素酶）能够将纤维素转化为 可发酵糖 这个转变过程的一部分是 纤维素颗粒变成更小的颗粒。 这种碎片化 酶促反应是酶促反应中最不为人所知的方面之一。 水解 本次调查的目的是分析和 数学模型的碎裂过程与 纤维素的酶水解。 将开发的模型是 可用于评估使用纤维素酶用于 膳食纤维的生产，并提供更多的了解整体 生产可发酵糖的机制。

During enzymatic hydrolysis, cellulose particles are attacked by cellulases resulting in the production of fermentable sugars. However, prior to the production of significant amounts of fermentable sugars, considerable fragmentation of the initial cellulose particles occurs. Although several investigators have reported on this phenomena, there has been very little effort at assessing the rate and extent of fragmentation, nor have there been analyses of the influence of cellulose structural features and cellulase characteristics on the rate and extent of fragmentation. The purpose of this investigation is to experimentally and mathematically analyze cellulose fragmentation. The influence of mean particles size, cellulosepore volume and crystallinity, and the characteristics of cellulases of Thermomonospora fusca on the rate and extent of fragmentation will be analyzed. Cellulose is the most abundant carbohydrate produced by the biosphere. It is a major component of plant cell walls. In addition, cellulose represents 40 percent of the municipal solid waste generated across the country. Over the last 10 years there has been considerable interest in taping these renewable resources for industrial chemicals and foods. Part of this interest is stimulated by the relatively low cost of cellulosic materials. A number of microorganisms produce enzymes (cellulases) that are capable of converting cellulose to fermentable sugars. Part of this conversion process is the break-up of cellulose particles into smaller particles. This fragmentation process is one of the least understood aspects of enzymatic hydrolysis. The purpose of this investigation is to analyze and to mathematically model the fragmentation process associated with the enzymatic hydrolysis of cellulose. The model to be developed would be useful for assessing the feasibility of using cellulases for the production of dietary fibers and provide more insight into the overall mechanism for producing fermentable sugars.