Use of An Insertional Mutant of Arabidopsis to Study Trichome Formation

使用拟南芥插入突变体研究毛状体形成

• 批准号: 8801949
• 负责人: Michael Marks
• 金额: $ 23.84万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-15 至 1992-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8801949&HistoricalAwards=false
• 关键词: Use; Insertional; Mutant; Arabidopsis; Study

The long term goal of this proposal is to obtain an understanding of the molecular processes involved in the differentiation of an epidermal cell into a plant hair (trichome). The specific objectives include: 1) Isolate and characterize a gene required for elaboration of stem trichomes on Arabiopsis. 2) Explore the utility of the trichome gene as a marker for transformation. 3) Isolate and characterize other trichome specific genes. The starting point for this work is a mutant of Arabidopsis that lacks stem trichomes. This mutant was isolated from a population of plants that were transformed with Agrobacterium. Because the mutation is most likely induced by a T-DNA insert, the isolation of the trichome gene will be facilitated by a T-DNA probe. The significance is three fold. First, the differentiation of an epidermal cell into a trichome provides a relatively simple model for cell differentiation. Second, since trichomes are associated with important traits such as insect resistance, salt tolerance, and cotton quality, the understanding of trichome formation on Arabidopsis may provide information on how trichomes of crop species might be manipulated for crop improvement. Finally, the stem trichome gene may function as a visible transformation marker. %%% Dr. Marks is studying the differentiation of an epidermal cell into a plant hair (trichome). This provides a relatively simple model for studying cell differentiation. Since trichomes are associated with important traits such as insect resistance, salt tolerance, and cotton quality, the understanding of trichome formation on Arabidopsis (a plant more amenable to study than commercially important crops) may provide information on how trichomes of crop species might be manipulated for crop improvement.

该提案的长期目标是了解表皮细胞分化为植物毛发（毛状体）所涉及的分子过程。 具体目标包括： 1) 分离并鉴定拟南芥茎毛细化所需的基因。 2）探索毛状体基因作为转化标记的效用。 3) 分离和表征其他毛状体特异性基因。 这项工作的起点是缺乏茎毛的拟南芥突变体。 该突变体是从用农杆菌转化的植物群体中分离出来的。 由于突变很可能是由 T-DNA 插入物诱导的，因此 T-DNA 探针将有助于毛状体基因的分离。 其意义有三方面。 首先，表皮细胞分化为毛状体为细胞分化提供了相对简单的模型。 其次，由于毛状体与抗虫性、耐盐性和棉花质量等重要性状相关，因此对拟南芥毛状体形成的了解可以提供有关如何操纵作物物种毛状体以改善作物的信息。 最后，茎毛状体基因可以作为可见的转化标记。 %%% Marks 博士正在研究表皮细胞分化为植物毛发（毛状体）。 这为研究细胞分化提供了一个相对简单的模型。由于毛状体与抗虫性、耐盐性和棉花质量等重要性状相关，因此对拟南芥（一种比商业上重要作物更适合研究的植物）毛状体形成的了解可以提供有关如何操纵作物物种毛状体以改善作物的信息。