Use of an Arabidopsis Mutant to Study the Induction of Plant Cell Differentiation

使用拟南芥突变体研究植物细胞分化的诱导

• 批准号: 9118306
• 负责人: Michael Marks
• 金额: $ 26.25万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118306&HistoricalAwards=false
• 关键词: Use; Arabidopsis; Mutant; Study; Induction

The objective of this proposal is to expand the analysis of a gene that is required for the initiation of trichome differentiation. This gene, GL1, was isolated and initially characterized during the previous granting period. It was found that the predicted polypeptide encoded by GL1 is homologous to the Myb class of transcriptional activators. Interestingly, the gene appears to be expressed in stipules, which develop at the base of the leaves, but not in leaf primordia where trichomes develop. This result suggests that GL1 may be involved in the production of a diffusible signal that activates trichome formation. Experiments are proposed to confirm or negate the stipule-derived, trichome morphogen hypothesis. The PI also examine the mechanism behind the reduction in trichome formation that is found in plants that contain a 35S-GL1 gene. Other experiments are designed to identify the sequences that mediate the temporal and spatial regulation of GL1, to begin to identify genes that encode proteins that interact with GL1 promoter or that directly interact with the GL1 protein. In addition, experiments are planned to determine if GL1 is regulated appropriately in other plant species and to begin the characterization of other members of the Arabidopsis myb gene family.

本建议的目的是扩大对一个 毛状体起始所需的基因 分化 这个基因GL 1被分离出来， 在上一个授予期间的特点。 发现 GL 1编码的预测多肽与 Myb类转录激活因子。 有趣的是 基因似乎在托叶中表达，托叶发育在 在叶基部，但不在叶原基的地方， 开发. 这一结果表明，GL 1可能参与了 产生一种可扩散的信号，激活毛状体 阵 提出实验来证实或否定 托叶衍生，毛状体形态发生假说。 PI也 研究毛状体形成减少背后的机制 在含有35 S-GL 1基因的植物中发现。 其他 设计实验来鉴定介导 GL 1的时空调控，以开始识别 编码与GL 1启动子相互作用的蛋白质的基因，或 直接与GL 1蛋白相互作用。 此外，本发明还提供了一种方法， 计划进行实验以确定GL 1是否受到调节 适当地在其他植物物种和开始 拟南芥myb基因其他成员的鉴定 家人