Acquisition of a Preparative Process HPLC

我们正在请求支持，以获得制备性的高效液相色谱。有了这个装置，这里大量使用的发酵装置设施得到了补充和扩展。细胞将被培养、收集、破碎，并使用所需的高效液相仪器，其内容物可以快速且高分辨率地进行分级。扩展后的能力将大大加快联邦支持的几个项目的进度。本文所描述的项目涉及厌氧细菌的产氢和代谢机制、非血红素铁氧化还原蛋白的相互转化以及与电子传递和氢代谢有关的人工酶的可能合成、甲烷合成和二氧化碳代谢、光合作用、生物发光以及利用种间氢转移和循环的厌氧菌的生长。

We are requesting support to obtain a preparative HPLC. With this apparatus, the extensive and heavily-used fermentation plant facilities in operation here are complemented and extended. Cells will be grown, collected, broken and, with the HPLC apparatus requested, their contents can be fractionated quickly and with high resolution. The extended capability will greatly accelerate progress in several Federally-supported projects. Projects described herein involve the mechanism of hydrogen production and metabolism in anaerobic bacteria, interconversions of non-heme iron-containing ferredoxins and the possible synthesis of artificial enzymes concerned with electron transport and hydrogen metabolism, methanogenesis and carbon dioxide metabolism, photosynthesis, bioluminescence and growth of anaerobes utilizing interspecies hydrogen transfer and cycling.