Acquisition of a Preparative Process HPLC

获得制备工艺 HPLC

• 批准号: 8803433
• 负责人: Harry Peck
• 金额: $ 6.99万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803433&HistoricalAwards=false
• 关键词: Acquisition; Preparative; Process; HPLC

We are requesting support to obtain a preparative HPLC. With this apparatus, the extensive and heavily-used fermentation plant facilities in operation here are complemented and extended. Cells will be grown, collected, broken and, with the HPLC apparatus requested, their contents can be fractionated quickly and with high resolution. The extended capability will greatly accelerate progress in several Federally-supported projects. Projects described herein involve the mechanism of hydrogen production and metabolism in anaerobic bacteria, interconversions of non-heme iron-containing ferredoxins and the possible synthesis of artificial enzymes concerned with electron transport and hydrogen metabolism, methanogenesis and carbon dioxide metabolism, photosynthesis, bioluminescence and growth of anaerobes utilizing interspecies hydrogen transfer and cycling.

我们请求支持获得制备型高效液相色谱。有了这个装置，这里广泛和大量使用的发酵厂设施得到了补充和扩展。细胞将被培养、收集、破碎，并使用要求的高效液相色谱仪，其内容物可以快速、高分辨率地分离。扩展的能力将大大加快几个联邦政府支持项目的进展。本文所描述的项目包括厌氧细菌的产氢和代谢机制，非血红素含铁氧化还原蛋白的相互转化以及与电子传递和氢代谢有关的人工酶的可能合成，甲烷生成和二氧化碳代谢，光合作用，厌氧菌利用种间氢转移和循环的生物发光和生长。