Acquisition of a Preparative Process HPLC

我们请求支持以获得制备型HPLC。 与 这种设备，广泛和大量使用的发酵， 在这里运行的工厂设施得到补充和扩展。 细胞将生长、收集、破碎，并使用HPLC 仪器要求，其内容物可以迅速分馏 并且具有高分辨率。 扩展后的能力将大大加快 联邦政府支持的项目。 本文所述项目 涉及氢的产生和代谢机制， 厌氧细菌，非血红素铁的相互转化 铁氧还蛋白和人工酶的可能合成 与电子传递和氢代谢有关， 甲烷生成和二氧化碳代谢，光合作用， 利用种间生物发光和厌氧菌生长 氢转移和循环。

We are requesting support to obtain a preparative HPLC. With this apparatus, the extensive and heavily-used fermentation plant facilities in operation here are complemented and extended. Cells will be grown, collected, broken and, with the HPLC apparatus requested, their contents can be fractionated quickly and with high resolution. The extended capability will greatly accelerate progress in several Federally-supported projects. Projects described herein involve the mechanism of hydrogen production and metabolism in anaerobic bacteria, interconversions of non-heme iron-containing ferredoxins and the possible synthesis of artificial enzymes concerned with electron transport and hydrogen metabolism, methanogenesis and carbon dioxide metabolism, photosynthesis, bioluminescence and growth of anaerobes utilizing interspecies hydrogen transfer and cycling.