Molecular Topology and Development in a Photosynthetic Membrane System

光合膜系统的分子拓扑和发育

• 批准号: 8803649
• 负责人: R. Clinton Fuller
• 金额: $ 15万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-15 至 1990-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8803649&HistoricalAwards=false
• 关键词: Molecular; Topology; Development; Photosynthetic; Membrane

The objective of this research is to further the understanding of the structure and function of the photosynthetic membrane system in the evolutionarily "primitive" prokaryote, Chloroflexus aurantiacus. The relative simplicity of its reaction center and light- harvesting antenna systems make Chloroflexus an excellent model system for the study of photosynthetic energy capture and the genetic regulation and assembly of its photosynthetic apparatus. These experiments are a continuation of previous work defining the architecture of the photosynthetic apparatus. In addition, a series of experiments applying recombinant DNA techniques will isolate genes encoding the photosynthetic apparatus in Chloroflexus. These isolated genes will be used for analyzing both the structure of the photosynthetic apparatus and its regulation of assembly. Application of recently developed genetic techniques has resulted in new approaches for studying regulation of assembly of bacterial photosynthetic membranes. Such experiments have largely confirmed the observations of earlier workers, in that assembly responds to light and oxygen levels. The exact mechanisms for this control have not yet been clarified, but it is clear that several photosynthetic components are under separate regulatory control. Current information indicates three levels of regulation. One regulatory mechanism appears unique to the Chloroflexus photosynthetic apparatus: post-translational protein processing. This mechanism may be involved in regulation of chlorosome assembly. Such a processing mechanism predicts that the several chlorosome polypeptides are encoded on a single message, translated into a single polypeptide, then cleaved. The genetic analysis proposed in this project will provide a direct test of the protein- processing hypothesis by determining the structure of the genes encoding the chlorosome polypeptides.

本研究的目的是进一步 了解的结构和功能的 光合膜系统的进化 “原始”原核生物，橙黄绿弯藻。 的 它的反应中心相对简单， 收获天线系统使Chloroflexus成为一个优秀的 光合能量捕获研究的模型系统 以及其光合作用的遗传调控和组装 设备. 这些实验是以前的实验的延续。 定义光合作用的结构 设备. 此外，还进行了一系列实验， 重组DNA技术将分离出编码 绿弯藻光合器的研究 这些孤立 基因将被用于分析两个结构的 光合机构及其组装的调节。 最近开发的遗传技术的应用， 导致了新的研究方法， 细菌光合膜的组装。 等 实验在很大程度上证实了 早期的工人，在该组件响应光， 氧气水平 这种控制的确切机制有 尚未澄清，但很明显， 光合作用组分处于单独的调控之下， 控制 目前的信息表明， 调控 一种监管机制似乎是独特的， 绿弯藻光合机构的翻译后功能 蛋白质加工 这一机制可能与 调节叶绿体组装。 这样的处理 机制预测，几种叶绿体多肽 都被编码在一条信息上， 多肽，然后切割。 中提出的遗传分析 这个项目将提供一个直接的蛋白质测试- 处理假设通过确定的结构， 编码叶绿体多肽的基因。