Biological Synthesis and Material Properties of Bacterial Polyesters

细菌聚酯的生物合成及材料性能

• 批准号: 9507153
• 负责人: R. Clinton Fuller
• 金额: $ 27.5万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9507153&HistoricalAwards=false
• 关键词: Biological; Synthesis; Material; Properties; Bacterial

; R o o t E n t r y F J2B C o m p O b j b W o r d D o c u m e n t H O b j e c t P o o l I2B I2B 4 @ @ A B C D E F G H I J F Microsoft Word 6.0 Document MSWordDoc Word.Document.6 ; }' ^ t |L t |D u q |H u h ~ t@ F F Pj j j j u N ~ u F Pj j j j u F Pj j j ~ u + U F P W t L t 6 6L 6 6J F P u F P F P ~ u Q ^MM Õ M U t. ^ G ; 9507153 Fuller The major objective of the research project is to continue our innovative program on the study of the synthesis, material properties, containment and degradation of microbial thermoplastics. This program has involved the closely coordinated activities of three Principal Investigators, PIs, including a polymer chemist, a biochemist and a microbiologist. In the studies on the containment and degradation of poly-(-hydroxyalkanoates (PHAs), the inclusion bodies or PHA granules in Pseudomonas oleovorans, the bacterium of choice, have been clearly shown to contain at least five proteins, tow of which are polymerases, one a depolymerase, and two that have a unique paracrystalline array lattice-like structure. We will particularly concentrate on the structure and function of these latter two structural polypeptides. In the polymer chemistry studies on biosynthesis and materials properties studies, an extensive array of new microbial polyesters has been produced and examined, including flexible homopolymers, elastomeric and adhesive copolymers, functionalized polymers and chemically modifi ed bacterial polyesters with specific materials properties. All of these have been made available from several, biologically diverse microorganisms. In addition, emphasis will be pursued investigating the mechanisms, control processes and the enzymology of both intra- and extra-cellular PHA depolymerase enzyme systems. This research program is being supported jointly by the Division of Molecular and Cellular Biosciences (Directorate fmr Biosciences) and the Division for Materials Research (Directorate for Mathematical and Physical Sciences. %%% The major objective of the research project is to continue our innovative program on the study of the synthesis, material properties, containment and degradation of microbial thermoplastics. This program has involved the closely coordinated activities of three Principal Investigators, PIs, including a polymer chemist, a biochemist and a microbiologist. In the studies on the containment and degradation of poly-(-hydroxyalkanoates (PHAs), the inclusion bodies or PHA granules in Pseudomonas oleovorans, the bacterium of choice, have been clearly shown to contain at least five proteins, tow of which are polymerases, one a depolymerase, and two that have a unique paracrystalline array lattice-like structure. We will particularly concentrate on the structure and function of these latter two structural polypeptides. In the polymer chemistry studies on biosynthesis and materials properties studies, an extensive array of new microbial polyesters has been produced and examined, including flexible homopolymers, elastomeric and adhesive copolymers, functionalized polymers and chemically modified bacterial polyesters with specidic materials properties. All of these have been made available from several, biologically diverse microorganisms. In addition, emphasis will be pursued investigating the mechanisms, control processes and the enzymology of both intra- and extra-cellular PHA depolymerase enzyme systems. *** ; Oh +' 0 S u m m a r y I n f o r m a t i o n ( ? $ H l D h R:\WWUSER\TEMPLATE\NORMAL.DOT Fuller, R.C. Robert Uffen Shelley A. Graves @ i @ 1B @ W8F2B @ G Microsoft Word 6.0 2 ; e = e H j j j j j j j 1 # % % % # H

;​R o o t E n t y F J2B C o m p o b j b W o r d d o c u m e n t H O b j e c t P O O l I2B I2B4 @ @ a * b * c * d * I * jF Microsoft Word 6.0文档MSWordDoc。6;​}' t |L t |D u q |H u H ~ t@ F Pj j j j j u N ~ u F Pj j j j j F Pj j j j ~ u + u F P W t L t 6 6L 6 6J F P u F P P P ~ u q ^MM Õ M u t. ^ G；该研究项目的主要目标是继续我们在微生物热塑性塑料的合成、材料特性、密封和降解研究方面的创新计划。该项目涉及三位首席研究员（pi）的密切协调活动，包括一位聚合物化学家、一位生物化学家和一位微生物学家。在对聚羟基烷酸酯（PHAs）的抑制和降解的研究中，选择的橄榄假单胞菌（Pseudomonas oleovorans）中的包涵体或PHA颗粒已被清楚地表明含有至少五种蛋白质，其中两种是聚合酶，一种是解聚合酶，另外两种具有独特的副晶阵列晶格状结构。我们将特别关注后两种结构多肽的结构和功能。在生物合成和材料性能的高分子化学研究中，大量新的微生物聚酯被生产和研究，包括柔性均聚物、弹性体和粘接共聚物、功能化聚合物和具有特定材料性能的化学改性细菌聚酯。所有这些都可以从几种生物多样性的微生物中获得。此外，重点将继续调查机制，控制过程和酶学的细胞内和细胞外的PHA解聚合酶系统。该研究项目由分子和细胞生物科学部（fmr生物科学部）和材料研究部（数学和物理科学部）联合支持。该研究项目的主要目标是继续我们在微生物热塑性塑料的合成、材料特性、密封和降解研究方面的创新计划。该项目涉及三位首席研究员（pi）的密切协调活动，包括一位聚合物化学家、一位生物化学家和一位微生物学家。在对聚羟基烷酸酯（PHAs）的抑制和降解的研究中，选择的橄榄假单胞菌（Pseudomonas oleovorans）中的包涵体或PHA颗粒已被清楚地表明含有至少五种蛋白质，其中两种是聚合酶，一种是解聚合酶，另外两种具有独特的副晶阵列晶格状结构。我们将特别关注后两种结构多肽的结构和功能。在生物合成和材料性能的高分子化学研究中，大量新的微生物聚酯被生产和研究，包括柔性均聚物、弹性体和粘接共聚物、功能化聚合物和具有特定材料性能的化学改性细菌聚酯。所有这些都可以从几种生物多样性的微生物中获得。此外，重点将继续调查机制，控制过程和酶学的细胞内和细胞外的PHA解聚合酶系统。*** ;哦，我是说，我是说，我是说，我是说，我是说，我是说，我是说，我是说，我是说，我是说，​$ H l D H R:\WWUSER\TEMPLATE\NORMAL。点 丰满,R.C. Robert Uffen Shelley A. Graves @ i @ 1B @ W8F2B @ G Microsoft Word 6.0 2.0；​e = e H j j j j j j j 1 # % % % # H