Maintenance of Escherichia coli K-12 Stock Culture Collection

大肠杆菌库存中心保存了 大约7,000个基因上确定的细菌储备培养物， E.大肠杆菌菌株K-12，并应要求分发约5000 这些变种人的培养。 请求来自 微生物领域的科学研究人员和教师 遗传学、分子生物学、生物化学、生物物理学、医学 研究，重组DNA技术和相关领域的人， 在学术和政府实验室工作，非营利 研究机构、工业实验室和重组DNA 事务所 库存中心将历史信息存档， E.杆菌K-12 同时也是身份信息的中心 和这些生物的基因图谱。 股票中心 策展人芭芭拉·巴赫曼博士出版了E.杆菌 K-12连锁图和E.大肠杆菌突变体。 中心 还协调基因符号、等位基因编号 和菌株名称。 为了促进上述职能，在授予期间， 中心将计算其广泛的记录和搜索 程序. 新的股票将被添加到收藏中， 随着新类型的突变体被分离出来， 遗传分析技术的发展。

The Escherichia coli Stock Center maintains a collection of around 7,000 genetically defined stock cultures of the bacterium, E. coli strain K-12, and distributes upon request about 5000 cultures of these mutants each year. Requests come from scientific investigators and teachers in the fields of microbial genetics, molecular biology, biochemistry, biophysics, medical research, recombinant DNA technology and related fields who are working in academic and governmental laboratories, non-profit research institutes, industrial laboratories and recombinant DNA firms. The Stock Center archives information on the histories, derivations and genotypes of mutant derivatives of E. coli K-12 and also acts as a center for information on the identification and genetic mapping of these organisms. The Stock Center's curator, Dr. Barbara Bachmann, publishes revisions of the E. coli K-12 linkage map and pedigrees of E. coli mutants. The Center also coordinates the assignment of gene symbols, allele numbers and strain designations. To facilitate the above functions, during this award the Center will computerize its extensive records and search procedures. New stocks will be added to the collection in response to needs as new types of mutants are isolated and new techniques of genetic analysis are developed.