Maintenance of Escherichia coli K-12 Stock Culture Collection

大肠杆菌 K-12 储存培养物保藏的维护

• 批准号: 8807021
• 负责人: Barbara Bachmann
• 金额: $ 188.84万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-15 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8807021&HistoricalAwards=false
• 关键词: Maintenance; Escherichia; coli; 12; Stock

The Escherichia coli Stock Center maintains a collection of around 7,000 genetically defined stock cultures of the bacterium, E. coli strain K-12, and distributes upon request about 5000 cultures of these mutants each year. Requests come from scientific investigators and teachers in the fields of microbial genetics, molecular biology, biochemistry, biophysics, medical research, recombinant DNA technology and related fields who are working in academic and governmental laboratories, non-profit research institutes, industrial laboratories and recombinant DNA firms. The Stock Center archives information on the histories, derivations and genotypes of mutant derivatives of E. coli K-12 and also acts as a center for information on the identification and genetic mapping of these organisms. The Stock Center's curator, Dr. Barbara Bachmann, publishes revisions of the E. coli K-12 linkage map and pedigrees of E. coli mutants. The Center also coordinates the assignment of gene symbols, allele numbers and strain designations. To facilitate the above functions, during this award the Center will computerize its extensive records and search procedures. New stocks will be added to the collection in response to needs as new types of mutants are isolated and new techniques of genetic analysis are developed.

大肠杆菌库存中心保留了大约7,000种基因定义的大肠杆菌K-12菌株的库存培养物，并应要求每年分发约5000种这些突变体的培养物。请求来自微生物遗传学、分子生物学、生物化学、生物物理学、医学研究、重组DNA技术及相关领域的科学研究人员和教师，他们在学术和政府实验室、非营利性研究机构、工业实验室和重组DNA公司工作。库存中心将有关E.ColiK-12突变衍生物的历史、衍生和基因类型的信息存档，并作为这些生物的鉴定和基因图谱的信息中心。该中心的馆长芭芭拉·巴赫曼博士发表了大肠杆菌K-12连锁图谱的修订版和大肠杆菌突变的系谱。该中心还协调基因符号、等位基因编号和菌株命名的分配。为了促进上述功能，在本次颁奖期间，中心将使其广泛的记录和检索程序计算机化。随着新类型突变的分离和新的遗传分析技术的开发，将根据需要增加新的种群。