Maintenance of Escherichia coli K-12 Stock Culture Collection

大肠杆菌库存中心保存着大约7000种基因确定的大肠杆菌菌株K-12的库存培养物，并根据要求每年分发大约5000种这些突变体的培养物。请求来自微生物遗传学、分子生物学、生物化学、生物物理学、医学研究、重组DNA技术和相关领域的科研人员和教师，他们在学术和政府实验室、非营利研究机构、工业实验室和重组DNA公司。该库存中心存档了大肠杆菌K-12的历史、衍生和突变衍生物的基因型信息，并作为这些生物鉴定和遗传图谱的信息中心。库存中心的馆长芭芭拉·巴赫曼博士出版了大肠杆菌K-12连锁图谱的修订版和大肠杆菌突变体的系谱。中心还协调基因符号、等位基因数和菌株名称的分配。为了促进上述职能，在此期间，中心将使其广泛的记录和检索程序电脑化。随着新型突变体的分离和新的遗传分析技术的发展，将根据需要增加新的品种。

The Escherichia coli Stock Center maintains a collection of around 7,000 genetically defined stock cultures of the bacterium, E. coli strain K-12, and distributes upon request about 5000 cultures of these mutants each year. Requests come from scientific investigators and teachers in the fields of microbial genetics, molecular biology, biochemistry, biophysics, medical research, recombinant DNA technology and related fields who are working in academic and governmental laboratories, non-profit research institutes, industrial laboratories and recombinant DNA firms. The Stock Center archives information on the histories, derivations and genotypes of mutant derivatives of E. coli K-12 and also acts as a center for information on the identification and genetic mapping of these organisms. The Stock Center's curator, Dr. Barbara Bachmann, publishes revisions of the E. coli K-12 linkage map and pedigrees of E. coli mutants. The Center also coordinates the assignment of gene symbols, allele numbers and strain designations. To facilitate the above functions, during this award the Center will computerize its extensive records and search procedures. New stocks will be added to the collection in response to needs as new types of mutants are isolated and new techniques of genetic analysis are developed.