Maintenance of Escherichia coli K-12 Genetic Stock Culture Collection

该奖项更新了NSF对E.大肠杆菌遗传储备中心（CGSC）。CGSC提供E.大肠杆菌的研究人员和教育工作者在生物学的各个领域。CGSC收集了超过10，000种土培大肠杆菌K-12菌株的突变衍生物，每年分发约5，000种培养物。 在其30年的历史中，已传播了13万多种文化。随着对大肠杆菌的组织和功能的详细了解的进展，对目前保藏的菌株以及将要添加的菌株的需求预计将增加。coli基因组。E.大肠杆菌仍然是一种重要的模式生物，从遗传学和生物化学的角度来看，可能是研究得最好的模式生物，并且在其他生物的遗传操作中充当关键工具。用户包括在学术和政府实验室、非营利研究机构和各种工业实验室工作的研究人员，所有这些人都依赖CGSC获得记录良好的菌株以及有关生物体和收集内容的在线信息。菌株和信息也提供给大学，学院和K-12学校的各级教师和学生。作为其在研究界的角色的一部分，CGSC协调基因符号和等位基因编号的分配，并鼓励遵守公认的遗传命名法。

This award renews NSF support for operation of the E. Coli Genetic Stock Center (CGSC). The CGSC supplies mutant and wild-type strains of E. coli to researchers and educators in various fields of biology. CGSC maintains a collection of over 10,000 mutant derivatives of Eschericia coli strain K-12,and distributes about 5,000 cultures annually. Over its 30-year history, more than 130,000 cultures have been distributed. Demand for strains currently in the collection, as well as strains to be added, is expected to increase as the result of progress in understanding the details of the organization and function of the E. coli genome. E. coli remains an important model organism, perhaps the best-studied from the standpoint of genetics and biochemistry, and serves as a critical tool in the genetic manipulation of other organisms. Users include researchers working in academic and governmental laboratories, non-profit research institutes, and diverse industrial laboratories, all of who rely on the CGSC for well-documented strains and for online information about the organism and about the contents of the collection. Strains and information are also provided to teachers and students at all levels in universities, colleges, and K-12 schools. As part of it's role in the research community, the CGSC coordinates assignment of gene symbols and allele numbers and encourages adherence to accepted genetic nomenclature.