Maintenance of Escherichia coli K-12 Genetic Stock Culture Collection

该奖项重申了美国国家科学基金会对大肠杆菌遗传储备中心（CGSC）运作的支持。CGSC为生物学各个领域的研究人员和教育工作者提供突变型和野生型大肠杆菌菌株。CGSC收集了大肠杆菌K-12菌株的10,000多个突变衍生物，每年分发约5,000个培养物。在其30年的历史中，已经分发了13万多个文化。由于对大肠杆菌基因组的组织和功能细节的了解取得进展，对目前收集的菌株以及将要添加的菌株的需求预计会增加。大肠杆菌仍然是一种重要的模式生物，从遗传学和生物化学的角度来看，它可能是研究得最好的，并且是对其他生物进行遗传操作的关键工具。用户包括在学术和政府实验室、非营利性研究机构和各种工业实验室工作的研究人员，他们都依赖CGSC获得记录良好的菌株，以及关于该生物和收集内容的在线信息。菌株和信息也提供给大学、学院和K-12学校的各级教师和学生。作为其在研究界角色的一部分，CGSC协调基因符号和等位基因数的分配，并鼓励遵守公认的遗传命名法。

This award renews NSF support for operation of the E. Coli Genetic Stock Center (CGSC). The CGSC supplies mutant and wild-type strains of E. coli to researchers and educators in various fields of biology. CGSC maintains a collection of over 10,000 mutant derivatives of Eschericia coli strain K-12,and distributes about 5,000 cultures annually. Over its 30-year history, more than 130,000 cultures have been distributed. Demand for strains currently in the collection, as well as strains to be added, is expected to increase as the result of progress in understanding the details of the organization and function of the E. coli genome. E. coli remains an important model organism, perhaps the best-studied from the standpoint of genetics and biochemistry, and serves as a critical tool in the genetic manipulation of other organisms. Users include researchers working in academic and governmental laboratories, non-profit research institutes, and diverse industrial laboratories, all of who rely on the CGSC for well-documented strains and for online information about the organism and about the contents of the collection. Strains and information are also provided to teachers and students at all levels in universities, colleges, and K-12 schools. As part of it's role in the research community, the CGSC coordinates assignment of gene symbols and allele numbers and encourages adherence to accepted genetic nomenclature.