Maintenance of Escherichia coli K-12 Genetic Stock Culture Collection

该奖项为位于耶鲁大学的大肠杆菌遗传储备中心的运作提供了持续的支持。该库存中心保存着大约7000种基因确定的大肠杆菌K-12菌株的库存培养物。斯托克中心将这些菌株分发给全世界的科学家。请求来自微生物遗传学、分子生物学、生物化学、生物物理学、医学研究、生物技术和相关领域的研究人员和教师，他们在学术和政府实验室、非营利研究机构、工业实验室和生物技术公司工作。除了培养本身，库存中心还提供有关大肠杆菌K-12突变衍生物的历史、衍生和基因型的信息，以及大肠杆菌染色体上基因的鉴定和遗传定位。该库存中心还作为基因符号、等位基因数和菌株名称分配的协调点。在上次发放期间，许多信息功能已电脑化，大部分信息现在已联机提供给公众。在今后五年中，斯托克中心将继续为科学界提供服务，并将通过扩大电子数据库使其运作更有效率，以便研究人员不仅可以访问这些信息，而且可以在自己的终端上使用这些信息来推进他们的研究。此外，即将退休的主任芭芭拉·巴赫曼博士将在下一个奖励期间发表最后一份大肠杆菌基因图谱。

9315421 Berlyn This award provides continued support for the operation of the Escherichia coli Genetic Stock Center located at Yale University. The Stock Center maintains a collection of around 7,000 genetically defined stock cultures of E. coli strain K-12. The Stock Center distributes these strains to the world-wide community of scientists. Requests come from investigators and teachers in the fields of microbial genetics, molecular biology, biochemistry, biophysics, medical research, biotechnology and related fields who are working in academic and governmental laboratories, non-profit research institutes, industrial laboratories and biotechnology firms. In addition to the cultures themselves, the Stock Center provides information on the histories, derivations, and genotypes of mutant derivatives of E. coli K-12 and on the identification and genetic mapping of genes on the chromosome of E. coli. The Stock Center also serves as a point of coordination for the assignment of gene symbols, allele numbers and strain designations. During the last granting period, many of the information functions have been computerized and much of the information is now available on-line to the public. During the next five years, the Stock Center will continue its service to the scientific community and will make its operation more efficient by expanding the electronic databases so that investigators can not only access the information but also use it to advance their research at their own terminals. In addition, the retiring Director, Dr. Barbara Bachmann will publish one final genetic map of E. coli during the next award period.