Early Developmental Gene Regulation in Dictyostelium

盘基网柄菌早期发育基因调控

• 批准号: 8809026
• 负责人: Charles Singleton
• 金额: $ 25.08万
• 依托单位: Vanderbilt University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8809026&HistoricalAwards=false
• 关键词: Early; Developmental; Gene; Regulation; Dictyostelium

Proper development of eukaryotic organisms requires differential expression of a multitude of genes with precise timing and tissue selectivity. Dr. Singleton is utilizing the developmental program of the cellular slime mold, Dictyostelium discoideum, as a system to investigate the molecular details of differential gene expression and the developmental regulation of this expression. He has identified and characterized three groups of genes which show various forms of differential expression upon the onset of development or shortly thereafter. Two groups are deactivated upon initiation of development, and the mechanisms of the deactivation events are very different between the two groups. A third set of genes is induced as development begins and is transiently expressed during the first few hours of the program. Dr. Singleton's analysis of mutant strains unable to develop has led to subclassification of the genes into those that appear to be under the same regulatory system. He will continue to isolate the genomic clones corresponding to certain of these genes and utilize them to investigate the molecular events involved in their regulation. The sequences necessary for transcription and proper regulation will be defined, and the protein factors which mediate the regulatory events will be identified and characterized. A system for generating and identifying mutations present in the genes encoding the regulatory machinery will be developed. Finally, through mutagenesis of a selected gene, he will begin to address what role these genes play in the establishment of the developmental program. %%% These investigations should result in a more comprehensive picture of the complexity and the details of the regulatory events leading to the activation and establishment of development using a simple model system. They will provide significant insight into the molecular machinery that is utilized in the control processes of early development.

真核生物的正常发育需要大量基因以精确的时间和组织选择性进行差异表达。 Singleton 博士正在利用细胞粘菌（盘基网柄菌）的发育程序作为系统来研究差异基因表达的分子细节以及该表达的发育调控。 他鉴定并表征了三组基因，这些基因在发育开始时或发育后不久表现出各种形式的差异表达。 两个群体在发育开始时就失活，并且两个群体之间失活事件的机制非常不同。 第三组基因在发育开始时被诱导，并在程序的最初几个小时内短暂表达。 辛格尔顿博士对无法发育的突变株的分析导致将基因细分为似乎处于同一调控系统下的基因。 他将继续分离与其中某些基因相对应的基因组克隆，并利用它们来研究参与其调控的分子事件。 将定义转录和适当调节所需的序列，并且将鉴定和表征介导调节事件的蛋白质因子。 将开发一种用于生成和识别编码调节机制的基因中存在的突变的系统。最后，通过对选定基因的诱变，他将开始研究这些基因在发育程序的建立中发挥的作用。 %%% 这些调查应该使用简单的模型系统更全面地了解导致开发激活和建立的监管事件的复杂性和细节。他们将为早期开发控制过程中使用的分子机制提供重要的见解。