Engineering Creativity Award: Purification of Proteins from Hyperthermophilic Bacteria for Thermostability Studies

这项跨学科的研究项目涉及化学工程和生物学领域，涉及从超高温细菌中分离蛋白质。研究这些新微生物的生物分子需要创造性地优化生长、分离生物产品和分离蛋白质。利用亲和层析和高效液相色谱两种纯化方案的结合，在最近开发的高效液体亲和层析(HPLAC)技术中，将从嗜热菌和超嗜热菌中分离和纯化选定的蛋白质。在这种组合中，利用了亲和体系的选择性和液体体系的速度和高分辨率。HPLAC技术的使用有望消除与多步纯化相关的高蛋白损失。嗜热菌蛋白质的稳定性对于优化工业中的高温反应具有重要意义。

This interdisciplinary research project is concerned with the fields of chemical engineering and biology and involves isolation of proteins from hyperthermophilic bacteria. Study of biomolecules from these novel microorganisms requires creative approches to optimizing growth, separating bioproducts, and isolating proteins. Using a blend of two purification schemes, affinity chromatography and high liquid chromotography, in a recently developed technique - high performance liquid affinity chromatography (HPLAC) - selected proteins from thermophiles and hyperthermophiles will be isolated and purified. In this combination, advantage is taken of the selectivity of the affinity system and the speed and high resolution of the liquid system. The use of the HPLAC technique is expected to eliminate the high protein losses associated with multistep purification. The stability of proteins from hyperthermophilic bacteria is of great interest in optimizing high temperature reactions in industry.