Engineering Creativity Award: Purification of Proteins from Hyperthermophilic Bacteria for Thermostability Studies
Engineering Creativity Award: Purification of Proteins from Hyperthermophilic Bacteria for Thermostability Studies
批准号:
8811561
负责人:
Robert Kelly
金额:
$6.0万
依托单位:
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-09-01 至 1991-01-31
中文摘要
这项跨学科的研究项目涉及化学工程和生物学领域,涉及从超高温细菌中分离蛋白质。研究这些新微生物的生物分子需要创造性地优化生长、分离生物产品和分离蛋白质。利用亲和层析和高效液相色谱两种纯化方案的结合,在最近开发的高效液体亲和层析(HPLAC)技术中,将从嗜热菌和超嗜热菌中分离和纯化选定的蛋白质。在这种组合中,利用了亲和体系的选择性和液体体系的速度和高分辨率。HPLAC技术的使用有望消除与多步纯化相关的高蛋白损失。嗜热菌蛋白质的稳定性对于优化工业中的高温反应具有重要意义。
英文摘要
This interdisciplinary research project is concerned with the fields of chemical engineering and biology and involves isolation of proteins from hyperthermophilic bacteria. Study of biomolecules from these novel microorganisms requires creative approches to optimizing growth, separating bioproducts, and isolating proteins. Using a blend of two purification schemes, affinity chromatography and high liquid chromotography, in a recently developed technique - high performance liquid affinity chromatography (HPLAC) - selected proteins from thermophiles and hyperthermophiles will be isolated and purified. In this combination, advantage is taken of the selectivity of the affinity system and the speed and high resolution of the liquid system. The use of the HPLAC technique is expected to eliminate the high protein losses associated with multistep purification. The stability of proteins from hyperthermophilic bacteria is of great interest in optimizing high temperature reactions in industry.
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Populating a Radiocarbon Database of North American, Phase II
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财政年份:2016
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Populating a Radiocarbon Database of Western North America
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财政年份:2014
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负责人:Robert Kelly
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依托单位:
COLLABORATIVE RESEARCH: Exploiting microbial hyperthermophilicity to produce an industrial chemical
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批准号:1264052
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项目类别:Standard Grant
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资助金额:$25.0万
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财政年份:2013
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依托单位:
Doctoral Dissertation Improvement Grant: Testing Colonization Models in New England
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批准号:1342656
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财政年份:2013
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Human Occupation of the Bighorn Mountains
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批准号:0710868
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财政年份:2007
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负责人:Robert Kelly
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依托单位:
Functional Approaches for Annotating Secretome-bound Small ORFs in Microbial Genomes
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批准号:0730091
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项目类别:Standard Grant
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资助金额:$31.88万
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财政年份:2007
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负责人:Robert Kelly
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依托单位:
Collaborative Research: Biotransformations Near and Above 100°C: Hyperthermophilic Microorganisms and Enzymes for Bioenergy Conversion
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批准号:0617272
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项目类别:Standard Grant
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资助金额:$42.04万
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财政年份:2006
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负责人:Robert Kelly
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依托单位:
LATE PLEISTOCENE HUMAN OCCUPATION OF THE BIGHORN MOUNTAINS
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批准号:0514863
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:2005
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负责人:Robert Kelly
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依托单位:
Fluxes in Advective and Transition Boundary Layers
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批准号:0354819
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项目类别:Continuing Grant
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资助金额:$37.82万
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财政年份:2004
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负责人:Robert Kelly
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依托单位:
Biotransformations Near and Above 100 C: Mining Genomes of Hyperthermophiles for New Biocatalysts
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批准号:0317886
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项目类别:Continuing Grant
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资助金额:$40.0万
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财政年份:2003
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负责人:Robert Kelly
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依托单位:
Collaborative Research: Molecular and Engineering Studies of Extremely Thermophilic Xylose Isomerases
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批准号:0115734
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项目类别:Continuing Grant
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资助金额:$28.64万
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财政年份:2001
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负责人:Robert Kelly
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依托单位:
Biotransformations Near and Above 100 Degrees Celsius
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批准号:0000487
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项目类别:Continuing Grant
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资助金额:$24.99万
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财政年份:2000
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负责人:Robert Kelly
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依托单位:
Early Holocene Prehistory in the Intermontane West: Reinvestigation of the Pine Spring Site, Southwest Wyoming
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批准号:9973272
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项目类别:Standard Grant
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资助金额:$3.13万
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财政年份:1999
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负责人:Robert Kelly
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依托单位:
Engineering Foundation Conference on Enzyme Engineering XV, Oct. 15-19, 1999, Kona, Hawaii
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批准号:9904113
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项目类别:Standard Grant
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资助金额:$1.4万
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财政年份:1999
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Microfabrication Methods Applied to the Development of Rigorously Defined Occluded Region Corrosion Samples
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批准号:9811412
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项目类别:Continuing Grant
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资助金额:$34.72万
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财政年份:1998
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负责人:Robert Kelly
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依托单位:
Biological and Chemical Energetics of Deep Sea Subsurface Life: Evolutionary and Exobiological Implications
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批准号:9816492
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项目类别:Standard Grant
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资助金额:$23.5万
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财政年份:1998
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负责人:Robert Kelly
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依托单位:
Collaborative Research: Biomolecular and Engineering Studies of Extremely Thermophilic Xylose (Glucose) Isomerases
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批准号:9817067
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项目类别:Continuing Grant
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资助金额:$24.49万
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财政年份:1998
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负责人:Robert Kelly
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依托单位:
海外基金