Engineering Creativity Award: Purification of Proteins from Hyperthermophilic Bacteria for Thermostability Studies

这个跨学科的研究项目涉及的领域 包括分离蛋白质 嗜热菌的细菌 研究生物分子从这些 新的微生物需要创造性的方法来优化生长， 分离生物产品和分离蛋白质。 使用两种混合物 纯化方案、亲和层析和高液相色谱 色谱法，在最近开发的技术-高性能 液相亲和层析（HPRAC）-选择的蛋白质来自 嗜热菌和超嗜热菌将被分离和纯化。 在 这种组合的优点在于， 亲和系统和液体的速度和高分辨率 系统 HPLAC技术的使用有望消除高 与多步纯化相关的蛋白质损失。 稳定性 嗜热菌的蛋白质是非常感兴趣的， 优化工业中的高温反应。

This interdisciplinary research project is concerned with the fields of chemical engineering and biology and involves isolation of proteins from hyperthermophilic bacteria. Study of biomolecules from these novel microorganisms requires creative approches to optimizing growth, separating bioproducts, and isolating proteins. Using a blend of two purification schemes, affinity chromatography and high liquid chromotography, in a recently developed technique - high performance liquid affinity chromatography (HPLAC) - selected proteins from thermophiles and hyperthermophiles will be isolated and purified. In this combination, advantage is taken of the selectivity of the affinity system and the speed and high resolution of the liquid system. The use of the HPLAC technique is expected to eliminate the high protein losses associated with multistep purification. The stability of proteins from hyperthermophilic bacteria is of great interest in optimizing high temperature reactions in industry.