Biotransformations Near and Above 100 Degrees Celsius

接近或高于 100 摄氏度的生物转化

• 批准号: 0000487
• 负责人: Robert Kelly
• 金额: $ 24.99万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0000487&HistoricalAwards=false
• 关键词: Biotransformations; Near; Above; 100; Degrees

The objective of this project is to focus on enzymes of technical importance from two key organisms, Pyrococcus furiosus and Thermotoga maritima. Both of these have been well characterized by the Principal Investigators and are suitable models for this project. Basic and applied objectives are to be pursued. The project is divided into five main areas. Areas one to four focus on answering specific biochemical questions on four enzymes: chitinases, glycosyl transferases, prolidases, and aminoacylases. The fifth area addresses the optimization of the hyperthermophile gene expression in E. coli.

该项目的目标是重点研究来自两种关键生物体--狂热杆菌和海洋耐热杆菌的具有重要技术意义的酶。首席调查员已很好地说明了这两种模式的特点，它们都是这一项目的合适模式。将追求基本的和应用的目标。该项目分为五个主要领域。领域一至领域四集中于回答关于四种酶的特定生化问题：几丁质酶、糖基转移酶、脯氨酸酶和氨基酰基酶。第五部分是对嗜热菌基因在大肠杆菌中表达的优化。