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US-France Cooperative Research: Sex Cell Isolation and Characterization in Crop Plants

美法合作研究：作物性细胞分离和表征

基本信息

批准号：
8815251
负责人：
H. Lloyd Mogensen
金额：
$ 0.89万
依托单位：
Northern Arizona University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1989
资助国家：
美国
起止时间：
1989-02-01 至 1992-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=8815251&HistoricalAwards=false
关键词：
US France Cooperative Research Sex

项目摘要

This award will support collaborative research between Dr. Lloyd Mogensen, Northern Arizona University and Dr. Christian Dumas, University of Claude Bernard Lyon I, France. The research will focus on the isolation and characterization of male and female gametes from three important crop plants, corn (Zea mays), wheat ___ ____ (Triticum aestivum) and cabbage (Brassica oleracea). Isolation ________ ________ ________ ________ procedures, gamete membrane and protein studies, cytochemistry, stereology, and cryostorage studies will be carried out by the French research team. The portion of the cooperative research to be done at Northern Arizona University deals primarily with detailed structural analyses of isolated sex cells, specifically, computer-generated reconstructions and computer-assisted quantitative ultrastructure based upon complete serial ultrathin sections. The experience of the U.S. and French investigators in quantitative, three-dimensional ultrastructure and their past and present work on structural analyses of male and female gametes of several flowering plants, including corn and wheat, make the proposed cooperative research well suited for the two laboratories involved. A pilot study, already initiated, has demonstrated the feasibility of the collaborative investigation. A long-range goal of this research is to be able to control and manipulate the fertilization process for crop plant improvement.

该奖项将支持北亚利桑那大学的劳埃德·摩根森博士和法国克劳德伯纳德里昂一世大学的克里斯蒂安·杜马斯博士之间的合作研究。该研究将重点关注玉米（Zea mays）、小麦（Triticum aestivum）和卷心菜（Brassica oleracea）这三种重要农作物的雄性和雌性配子的分离和表征。分离 ________ ________ ________ ________ 程序、配子膜和蛋白质研究、细胞化学、体视学和冷冻保存研究将由法国研究小组进行。北亚利桑那大学合作研究的部分主要涉及分离性细胞的详细结构分析，特别是基于完整的连续超薄切片的计算机生成的重建和计算机辅助的定量超微结构。美国和法国研究人员在定量、三维超微结构方面的经验以及他们过去和现在对包括玉米和小麦在内的几种开花植物的雄性和雌性配子结构分析的工作，使得拟议的合作研究非常适合所涉及的两个实验室。一项已经启动的试点研究证明了合作调查的可行性。这项研究的长期目标是能够控制和操纵施肥过程以改良作物。