Cytological Analysis of Plastid Transmission in Medicago Sativa

苜蓿质体传播的细胞学分析

• 批准号: 9103658
• 负责人: H. Lloyd Mogensen
• 金额: $ 15.43万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-15 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9103658&HistoricalAwards=false
• 关键词: Cytological; Analysis; Plastid; Transmission; Medicago

The proposed study is part of an on-going project to determine the cellular basis of cytoplasmic inheritance in alfalfa. This species is one of a few in which there is a predominance of male plastid transmission to the offspring, and for which considerable genetic data are available. Plastid transmission is under genetic control, and genotypes have been characterized as strong,intermediate or weak male or females, according to their plastid transmission frequencies. To date the PI's laboratory has used quantitative, three dimensional ultrastructure, along with a DNA-specific fluorochrome, to analyze generative cells and sperm cells of strong and weak male lines. The first objective of the proposed research is to compare the egg cells of strong and weak females to determine whether egg composition before fertilization is a determining factor in plastid inheritance, as preliminary data suggest. Further studies will analyze fertilized eggs (zygotes) and early embryos in order to follow the specific fates of male and female plastids during these critical stages of embryogenesis. This research will complement other studies utilizing techniques of classical and molecular genetics, and promise to be invaluable for providing cytological explanations for observed patterns of cytoplasmic organelle inheritance in an economically important plant.

这项拟议的研究是一个正在进行的项目的一部分，该项目旨在确定紫花苜蓿细胞质遗传的细胞基础。这个物种是少数几个雄性叶绿体传递给后代的优势种之一，并且有相当多的遗传数据可用。叶绿体的传播受基因控制，根据叶绿体传播的频率，基因类型分为强、中、弱三种类型。到目前为止，PI的实验室使用了定量的三维超微结构，以及DNA特有的荧光色素，来分析强弱雄性品系的生殖细胞和精子细胞。这项拟议研究的第一个目标是比较强壮和虚弱雌性的卵细胞，以确定受精前的卵子组成是否像初步数据显示的那样，是决定叶绿体遗传的一个因素。进一步的研究将分析受精卵(受精卵)和早期胚胎，以跟踪这些胚胎发育的关键阶段中雄性和雌性质体的特定命运。这项研究将补充利用经典和分子遗传学技术的其他研究，并有望为观察到的经济重要植物的细胞质遗传模式提供宝贵的细胞学解释。