Solute Transport and Binding Kinetics in Affinity Chromatography

将研制一种新型光纤探头， 研究：（1）溶质通过支持基质的运动， (2)固定化配体与溶质结合的内在动力学。 探头是一根光纤，采用倏逝波激发 分子在纤维表面;电磁场是 波只穿透2000埃到周围的介质， 可以用来研究光学表面的分子 光纤 在项目的第二部分，波导将 涂覆有非常薄的支持基质膜， 感兴趣的溶质通过亲和柱的运动将 测定 这将允许分离质量传递， 直接测量的平衡效应。 因此， 载体基质的性质和配体连接的方法可以 要毫不含糊地确定。 这方面的知识将是相当大的 在亲和、色谱、分离技术中重要性， 生物技术的应用。 基于亲和性的分离是用于回收的重要技术 生物制品。 虽然研究广泛，但大多数 调查一直在宏观层面;例如，一些 研究使用了前沿分析或脉冲技术， 色谱理论 本项目将尝试研究 从微观上影响结合常数和传质速率 perspective.

A novel fiber optic probe will be developed and then used to investigate: (1) the movement of solutes through a support matrix and (2) the intrinsic kinetics of solute binding by immobilized ligands. The probe is an optical fiber employing an evanescent wave to excite molecules at the fiber surface; the electromagnetic field which is the wave penetrates only 2000 angstroms into the surrounding medium, and can be used to study the molecules at the surface of the optical fiber. In the second part of the project, the waveguide will be coated with a very thin film of a support matrix and the rate of movement of solutes of interest through an affinity column will be determined. This will allow separation of mass transfer and equilibrium effects by direct measurement. Thus, the influence of the nature of the support matrix and the method of ligand attachment can be unambiguously determined. This knowledge will be of considerable importance in affinity, chromatography, a separation technique with applications in biotechnology. Affinity-based separations are an important technique for the recovery of biological products. Though extensively studied, most investigations have been at the macroscopic level; for example, some studies have used frontal analysis or pulse techniques from chromatography theory. This project will attempt a study of factors affecting binding constants and mass transfer rates from a microscopic perspective.