Cephalopod Hemocyanins: Structure and Function

该项目的目的是了解结构，组装， 一个非常大的多亚基的生理功能 蛋白质，太平洋章鱼的血蓝蛋白，章鱼 dofleini 该功能大分子组合物是一种 非常大的多肽链的十聚体，每个质量为3.5 x 105道尔顿。 每个单体链折叠成七个 域，每个域携带氧结合位点。 的 来自c-DNA的单体链的氨基酸序列 库，正在确定整个序列。 比较 将提供一个不寻常的观点， 蛋白质进化，因为原始基因的串联拷贝 正在接受检查。 意图确定（或 域组）将被隔离， 将氧结合功能的差异与 序列，并确定不同的结构域可能 在装配过程中发挥作用。 如果有可能 重新关联一个或多个结构域已被 移除，氧结合将被分析，看看是什么。 所观察到的约束力保持合作性质的程度 在天然十聚体分子中。 一个共同的主题是，许多这些观察是伟大的 在整个十聚体和其 零件. 需要的是氧结合实验， 二聚体结构或十聚体，其中一个或多个 域类型丢失，以探测 在整体的运作中。 现在可以 可能 另一个有趣的方面是进化， 软体动物和节肢动物血蓝蛋白的产生 进化？ //

The aim of the project to understand the structure, assembly, and physiological function of a very large multi-subunit protein, the hemocyanin of the Pacific octopus, Octopus dofleini. The functional macromolecular assemblage is a decamer of very large polypeptide chains, each of mass 3.5 x 105 daltons. Each monomer chain is folded into seven domains, each of which carries an oxygen binding site. The amino acid sequence of the monomer chain from a c-DNA library, is being determined the entire sequence. Comparison of sequences between domains will provide an unusual view of protein evolution, since tandem copies of a primitive gene are being examined. Individual domains with the intention of determining (or groups of domains) will be isolated and attempts will be made to correlate differences in oxygen binding function with sequences, and to determine what role different domains may play in the assembly process. If it proves possible to reassociate subunits from which one or more domains have been removed, the oxygen binding will be analyzed to see what extent the binding retains to cooperative character observed in the native decameric molecule. A common theme is many of these observations is the great disparty in oxygen binding between the whole decamer and its parts. What is needed are oxygen binding experiments using either dimeric structure or decamers in which one or more domain types are missing, to probe the role of parts of the structure in the functioning of the whole. It may now be possible. Another aspect of interest is evolution, how did molluscan and arthropod hemocyanins arise and independently evolve? //