HEMOCYANINS; & CARBONIC ANHYDRASE,

血蓝蛋白；

• 批准号: 6444699
• 负责人: MARY HAMILTON
• 金额: $ 29.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444699
• 关键词: Invertebrata; biomedical resource; enzymes; hematology; proteins

The STEM is being used for mass measurement and the possible localization of specific groups by Au-labeling (carbohydrate or SH groups) in the study of the molluscan hemocyanins (Hc). The simplest of these decameric cylindrical macromolecules is ten times the size of the arthropodan hexameric Hc. The Hcs of marine gastropods consist of multiple decameric cylinders assembled into. di-decamers, and of particular interesi, td-decamers and higher arrays. Many other hemocyanins have been compared to try to discover wbat determines the preferred number of decamers for a particular organism. Several different methods of punP gigantea ying the hemocyamn, from P. were examined in the STEM for their quality. The hope is to be able to perform SAS experiments on them, similar to those described for CIpP (see Flanagan). - Carbonic anhydrase, is the enzyme used by oyster in shell formation. This has been purified and shown to be composed of 35kD subunits. Unlike the vertebrate complex which functions as a 30kD monomer, the snail enzyme is a complex of roughly 2MD. STEM mass measurements are being used to determine the number and spatial arrangement of subunits. Acety1cholinesterases from gastropods are also planned to be examined in the STEM.

STEM被用于质量测量， 通过Au标记（碳水化合物或SH）定位特定基团 组）的软体动物血蓝蛋白（Hc）的研究。 最简单的 这些十聚体圆柱形大分子的大小是 节肢动物六聚体Hc.海洋腹足类的Hcs包括 多个十室圆柱体组装成。 二-十聚体，和 特别是感兴趣的TD十聚体和更高的阵列。 许多其他 比较血蓝蛋白，试图发现WBAT决定了 特定生物体的十聚体的优选数目。 几 用不同的方法提取血蓝蛋白， 在STEM中检查其质量。 我们希望能够 对它们进行SAS实验，类似于对CipP所描述的实验 (see Flanagan）。 - 碳酸酐酶，是牡蛎用来 壳层形成 这已被纯化，并表明是由 35 kD亚基。 与脊椎动物复合体不同， 30 kD单体，蜗牛酶是一个大约2 MD的复合物。 STEM质量 测量被用来确定数量和空间 子单元的布置。 腹足类乙酰胆碱酯酶 也计划在STEM中进行检查。