Cephalopod Hemocyanins: Structure and Function

PI建议继续研究其结构，演变， 有限延伸的头足类血蓝蛋白及其功能 章鱼血蓝蛋白的最新工作和主要的新项目 包括鹦鹉螺和枪乌贼的血蓝蛋白。 最初，某些正在进行的章鱼血蓝蛋白研究将 完成。 这些实验包括利用 切割的亚基作为探索功能行为的工具。 详细分析了鹦鹉螺的亚基结构， 血蓝蛋白将承担，以提供那种 主要研究者为八达通开发的完整结构图。的 每种情况下涉及的多肽链的种类数，以及 将确定每个链中的功能单元的数量。 将评估装配中多种链类型的作用。 将进行有限的肽测序，以允许 Loligo和Nautilus单位与Octopus单位的对齐 以及腹足类血蓝蛋白中的那些。 如果时间和资金允许， 重新审视氧结合功能和变构 Loligo血蓝蛋白被提出，因为早期的研究表明 显著的氧键解离过程。 这个缓慢的过程 应该允许对血蓝蛋白解离进行第一次动力学研究 反应

The PI proposes to continue studies of the structure, evolution, and function of cephalopod hemocyanins with limited extension of recent work on Octopus hemocyanin and major new projects involving the hemocyanins of Nautilus and of the squid Loligo. Initially, certain ongoing studies of Octopus hemocyanin will be brought to completion. These include experiments utilizing cleaved subunits as tools in exploration of functional behavior. A detailed analysis of the subunit structure of the Nautilus and Loligo hemocyanins will be undertaken, to provide the kind of complete structural picture the PI has developed for Octopus. The number of kinds of polypeptide chains involved in each case, and the number of functional units in each chain will be determined. The roles of multiple chain types in assembly will be assessed. Limited peptide sequencing will be carried out in order to allow alignment of the Loligo and Nautilus units with those of Octopus and with those in gastropod hemocyanins. If time and funds permit, a re-examination of the oxygen-binding function and allostery of Loligo hemocyanin is proposed, since early studies indicated a remarkable oxygen-linked dissociation process. This slow process should allow the first kinetic studies on a hemocyanin dissociation reaction.