Cephalopod Hemocyanins: Structure and Function

头足类血蓝蛋白：结构和功能

• 批准号: 9119855
• 负责人: Kensal Van Holde
• 金额: $ 28.34万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119855&HistoricalAwards=false
• 关键词: Cephalopod; Hemocyanins; Structure; Function

The PI proposes to continue studies of the structure, evolution, and function of cephalopod hemocyanins with limited extension of recent work on Octopus hemocyanin and major new projects involving the hemocyanins of Nautilus and of the squid Loligo. Initially, certain ongoing studies of Octopus hemocyanin will be brought to completion. These include experiments utilizing cleaved subunits as tools in exploration of functional behavior. A detailed analysis of the subunit structure of the Nautilus and Loligo hemocyanins will be undertaken, to provide the kind of complete structural picture the PI has developed for Octopus. The number of kinds of polypeptide chains involved in each case, and the number of functional units in each chain will be determined. The roles of multiple chain types in assembly will be assessed. Limited peptide sequencing will be carried out in order to allow alignment of the Loligo and Nautilus units with those of Octopus and with those in gastropod hemocyanins. If time and funds permit, a re-examination of the oxygen-binding function and allostery of Loligo hemocyanin is proposed, since early studies indicated a remarkable oxygen-linked dissociation process. This slow process should allow the first kinetic studies on a hemocyanin dissociation reaction.

项目负责人建议继续研究头足类血蓝蛋白的结构、进化和功能，并有限扩展近期有关章鱼血蓝蛋白的工作以及涉及鹦鹉螺和鱿鱼 Loligo 血蓝蛋白的重大新项目。 最初，某些正在进行的章鱼血蓝蛋白研究将完成。 其中包括利用切割亚基作为探索功能行为的工具的实验。 我们将对 Nautilus 和 Loligo 血蓝蛋白的亚基结构进行详细分析，以提供 PI 为章鱼开发的完整结构图。将确定每种情况中涉及的多肽链的种类数量以及每条链中的功能单元的数量。 将评估多种链条类型在装配中的作用。 将进行有限的肽测序，以便将 Loligo 和 Nautilus 单位与章鱼和腹足动物血蓝蛋白的单位进行比对。 如果时间和资金允许，建议重新检查 Loligo 血蓝蛋白的氧结合功能和变构，因为早期研究表明存在显着的氧相关解离过程。 这个缓慢的过程应该可以对血蓝蛋白解离反应进行首次动力学研究。