HEMOCYANINS; & CARBONIC ANHYDRASE,
HEMOCYANINS; & CARBONIC ANHYDRASE,
批准号:
6308947
负责人:
MARY HAMILTON
金额:
$0.97万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-04-01 至 2001-03-31
中文摘要
STEM正被用于质量测量和可能的
通过金标记(碳水化合物或SH)定位特定基团
组)在软体动物血蓝蛋白(HC)的研究中。最简单的
这些十聚体圆柱形大分子的大小是
节肢动物六角体HC。海洋腹足类的HCS包括
多个十进制钢瓶组装成。Di-Decamers和Of
特别感兴趣的是,TD-Decamer和更高的阵列。许多其他人
已经比较了血蓝蛋白,试图发现WBAT决定了
特定生物体的首选分瓶器数量。几个
用不同的方法提取血蓝蛋白,分别为:
在STEM中对其质量进行检查。我们的希望是能够
在它们上执行SAS实验,类似于为CIPP描述的那些
(见弗拉纳根)。-碳酸脱水酶,是牡蛎在
贝壳的形成。它已经被提纯,并被证明是由
35kD亚基。不同于脊椎动物复合体,它的功能是
30kD的单体,蜗牛酶是一个约2mD的复合体。茎质量
测量被用来确定数量和空间
亚基的排列。来自腹足动物的乙酰胆碱酯酶是
还计划在STEM接受检查。
英文摘要
The STEM is being used for mass measurement and the possible
localization of specific groups by Au-labeling (carbohydrate or SH
groups) in the study of the molluscan hemocyanins (Hc). The simplest
of these decameric cylindrical macromolecules is ten times the size of
the arthropodan hexameric Hc. The Hcs of marine gastropods consist of
multiple decameric cylinders assembled into. di-decamers, and of
particular interesi, td-decamers and higher arrays. Many other
hemocyanins have been compared to try to discover wbat determines the
preferred number of decamers for a particular organism. Several
different methods of punP gigantea ying the hemocyamn, from P. were
examined in the STEM for their quality. The hope is to be able to
perform SAS experiments on them, similar to those described for CIpP
(see Flanagan). - Carbonic anhydrase, is the enzyme used by oyster in
shell formation. This has been purified and shown to be composed of
35kD subunits. Unlike the vertebrate complex which functions as a
30kD monomer, the snail enzyme is a complex of roughly 2MD. STEM mass
measurements are being used to determine the number and spatial
arrangement of subunits. Acety1cholinesterases from gastropods are
also planned to be examined in the STEM.
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会议论文
HEMOCYANINS; & CARBONIC ANHYDRASE,
-
批准号:6444699
-
项目类别:
-
资助金额:$29.31万
-
财政年份:2001
-
负责人:MARY HAMILTON
-
依托单位:
HEMOCYANINS; & CARBONIC ANHYDRASE,
-
批准号:6281344
-
项目类别:
-
资助金额:$0.41万
-
财政年份:1998
-
负责人:MARY HAMILTON
-
依托单位:
HEMOCYANINS & CARBONIC ANHYDRASE
-
批准号:6251693
-
项目类别:
-
资助金额:$0.84万
-
财政年份:1997
-
负责人:MARY HAMILTON
-
依托单位:
HEMOCYANINS & CARBONIC ANHYDRASE
-
批准号:5223589
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MARY HAMILTON
-
依托单位:--
海外基金