HEMOCYANINS; & CARBONIC ANHYDRASE,

血蓝蛋白；

• 批准号: 6308947
• 负责人: MARY HAMILTON
• 金额: $ 0.97万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308947
• 关键词: Invertebrata; biomedical resource; enzymes; hematology; proteins

The STEM is being used for mass measurement and the possible localization of specific groups by Au-labeling (carbohydrate or SH groups) in the study of the molluscan hemocyanins (Hc). The simplest of these decameric cylindrical macromolecules is ten times the size of the arthropodan hexameric Hc. The Hcs of marine gastropods consist of multiple decameric cylinders assembled into. di-decamers, and of particular interesi, td-decamers and higher arrays. Many other hemocyanins have been compared to try to discover wbat determines the preferred number of decamers for a particular organism. Several different methods of punP gigantea ying the hemocyamn, from P. were examined in the STEM for their quality. The hope is to be able to perform SAS experiments on them, similar to those described for CIpP (see Flanagan). - Carbonic anhydrase, is the enzyme used by oyster in shell formation. This has been purified and shown to be composed of 35kD subunits. Unlike the vertebrate complex which functions as a 30kD monomer, the snail enzyme is a complex of roughly 2MD. STEM mass measurements are being used to determine the number and spatial arrangement of subunits. Acety1cholinesterases from gastropods are also planned to be examined in the STEM.

STEM正被用于质量测量和可能的 通过金标记(碳水化合物或SH)定位特定基团 组)在软体动物血蓝蛋白(HC)的研究中。最简单的 这些十聚体圆柱形大分子的大小是 节肢动物六角体HC。海洋腹足类的HCS包括 多个十进制钢瓶组装成。Di-Decamers和Of 特别感兴趣的是，TD-Decamer和更高的阵列。许多其他人 已经比较了血蓝蛋白，试图发现WBAT决定了 特定生物体的首选分瓶器数量。几个 用不同的方法提取血蓝蛋白，分别为： 在STEM中对其质量进行检查。我们的希望是能够 在它们上执行SAS实验，类似于为CIPP描述的那些 (见弗拉纳根)。-碳酸脱水酶，是牡蛎在 贝壳的形成。它已经被提纯，并被证明是由 35kD亚基。不同于脊椎动物复合体，它的功能是 30kD的单体，蜗牛酶是一个约2mD的复合体。茎质量 测量被用来确定数量和空间 亚基的排列。来自腹足动物的乙酰胆碱酯酶是 还计划在STEM接受检查。