Transduction of Blue Light Signals in Higher Plants

摘要蓝光照射黄化豌豆幼苗后，加入三磷酸腺苷可显著降低质膜相关蛋白120kD的体外磷酸化能力。所描述的实验将a)提供光反应的完整光生物学表征；b)研究其光生物学特性与已知对蓝光的生理反应的相关性；c)生化地表征磷酸化变化；以及d)表征蛋白质本身。可测量的磷酸化变化发生在几秒钟内，PI将表明这四个领域的进展将阐明从蓝光感光到最终生理反应的一个或多个信号转导链的早期步骤。植物的生长受光的控制，光的数量和种类都是如此。某些植物的生长反应受到高能蓝光的调控。这项研究将研究植物感知光并将能量转化为植物功能的机制。

Abstract Blue Light irradiation of etiolated pea seedling strongly reduces the capacity of a 120 kD plasma membrane- associated protein for subsequent in vitro phosphorylation by added ATP. Experiments are described that will a) provide a complete photobiological characterization of the light reaction; b) investigate the correlation of its photobiological properties with known physiological responses to blue light; c) characterize the phosphorylation change biochemically; and d) characterize the protein itself. The measureable changes in phosphorylation occur in seconds, and the PI will show that progress in these four areas will elucidate early steps in one or more signal-transduction chains from blue light photoperception to final physiological response. Plant growth is controlled by light, both the amount and kind of light it receives. Certain plant growth responses are regulated by high energy blue light. This research will examine the mechanism by which plants perceive light and transform that energy into plant functions.