Biochemical and Molecular Investigations of the Transductionof Blue Light Signals in Arabidopsis thaliana

拟南芥蓝光信号转导的生化和分子研究

• 批准号: 9219256
• 负责人: Winslow Briggs
• 金额: $ 27万
• 依托单位: Carnegie Institution of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219256&HistoricalAwards=false
• 关键词: Biochemical; Molecular; Investigations; Transductionof; Blue

Blue light photoreception plays an important role in the regulation of higher plant development. Blue light photoreceptors mediate directional growth toward the light on the one hand (phototropism) and the extremely rapid inhibition of growth of stem elongation that is a part of the early de-etiolation response of emerging seedlings on the other. There is strong evidence that these two processes proceed through separate and independent signal transduction pathways. The goals of this research are: (1) to isolate, characterize, and obtain sequence information on a plasma membrane protein that is phosphorylated as a consequence of blue light irradiation, and that appears to be involved at an early stage in phototropism, and (2) to isolate, characterize, and obtain sequence information on a protein cross-reactive with several antibodies directed against animal intermediate filament proteins, a protein that may be involved in the rapid inhibition of growth. Arabidopsis thaliana mutants are available in both pathways, making Arabidopsis the organism of choice for these studies. It is expected that complete sequence information will be obtained on these two A. thaliana proteins involved respectively in two different blue light-induced signal transduction pathways. %%% Blue light is an important environmental signal in plant growth and development. It stimulates plants to grow toward light and regulates the elongation of young seedlings as they emerge from the soil. This research will take advantage of the power of molecular genetics to study the structure and role of two specific proteins involved in early stages of the response of plants to blue light. The results will be important contributions to our understanding of the molecular basis of plant responses to the environment.

蓝光接受在高等植物生长发育的调控中起着重要作用。蓝光感受器一方面介导向光的定向生长(向光性)，另一方面介导茎伸长生长的极快速抑制，这是出苗早期脱黄化反应的一部分。有强有力的证据表明，这两个过程通过不同和独立的信号转导途径进行。本研究的目标是：(1)分离、鉴定和获得由于蓝光照射而被磷酸化的质膜蛋白的序列信息，以及(2)分离、鉴定和获得与几种针对动物中间丝蛋白的抗体交叉反应的蛋白质的序列信息。一种可能参与快速抑制生长的蛋白质。拟南芥突变体在这两条途径上都是可用的，这使得拟南芥成为这些研究的首选有机体。预计将获得这两个分别参与两种不同蓝光诱导信号转导途径的拟南芥蛋白的完整序列信息。蓝光是植物生长发育中的重要环境信号。它刺激植物向着光生长，并调节幼苗从土壤中长出时的伸长。这项研究将利用分子遗传学的力量来研究两种特定蛋白质的结构和作用，这些蛋白质参与植物对蓝光反应的早期阶段。这一结果将为我们理解植物对环境反应的分子基础做出重要贡献。