Physiological and Molecular Studies of Phototropism in Arabidopsis thaliana

拟南芥向光性的生理和分子研究

• 批准号: 9601164
• 负责人: Winslow Briggs
• 金额: $ 54万
• 依托单位: Carnegie Institution of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9601164&HistoricalAwards=false
• 关键词: Physiological; Molecular; Studies; Phototropism; Arabidopsis

9601164 Briggs As sessile organisms, plants have evolved the capacity to respond to a wide range of environmental signals, one of the most important of which is light. Indeed, virtually every phase of a plant's development is regulated by its light environment. These collective responses are encompassed by the term photomorphogenesis. Since Koornneef et al. first described the hy (long hypocotyl) mutants of Arabidopsis, mutants have been central to photomorphogenesis research. The present award to study signal transduction in a blue light-activated system, phototropism, also relies heavily on the use of mutants. Dr. Brigg's laboratory has recently isolated a number of severe phototropism mutants in Arabidopsis thaliana designated nph for non-phototropic hypocotyl. They fall into four complementation groups, nph1-4. All five alleles of the first group, nph1, are deficient in or lack the phosphoprotein but show normal gravitropism, and it is the NPH1 gene that is focal point for this proposal. The working hypothesis is that NPH1 is the gene that encodes the phosphoprotein. As the weakest allele(nph1-2) shows altered spectral sensitivity for phototropism, NPH1 may itself be the photoreceptor. This research will contribute to extending our knowledge about the many ways in which light plays a role in plant growth and development.

9601164布里格斯作为固着生物，植物已经进化出对广泛的环境信号做出反应的能力，其中最重要的信号之一是光。 事实上，植物发育的每一个阶段都受到其光环境的调节。 这些集体反应被光形态建成这个术语所涵盖。 自Koornneef等人首次描述拟南芥hy（长下胚轴）突变体以来，突变体一直是光形态建成研究的核心。 目前研究蓝光激活系统（向光性）中信号转导的奖项也在很大程度上依赖于突变体的使用。 Brigg博士的实验室最近在拟南芥中分离出了一些严重的向光性突变体，命名为nph，表示非向光性下胚轴。 它们分为四个互补组，nph 1 -4。 第一组的所有五个等位基因nph 1都缺乏或缺乏磷蛋白，但显示正常的向重力性，并且NPH 1基因是该提议的焦点。 工作假设是NPH 1是编码磷蛋白的基因。 由于最弱的等位基因（nph 1 -2）显示出对向光性的光谱敏感性改变，NPH 1本身可能是光感受器。 这项研究将有助于扩大我们对光在植物生长和发育中发挥作用的许多方式的了解。