Blue light receptor cryptochrome-Intracellular localization and signal transduction

蓝光受体隐花色素-细胞内定位与信号转导

• 批准号: 15570031
• 负责人: YAMAMOTO Naoki
• 金额: $ 2.37万
• 依托单位: Ochanomizu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570031/
• 关键词: cryptochrome; photoreceptor; blue light; NLS; NES; nuclear import; nuclear export; signal transduction; イネ; 核輸送

Cryptochromes are blue light receptors regulating development in plants. Though Arabidopsis cryptochrome 1 (AtCRY1) has been reported to be localized in nucleus, this characteristic is not entirely conserved among those from a variety of plant species. In this study it was shown that most molecules of rice cryptochrome 1 is localized to nucleus but at least a small aliquot is localized to cytoplasm. To determine the relationship between intracellular localization and function of cryptochrome, we constructed a GFP-OsCRY1 gene and examined the activity in transgenic Arabidopsis plants (hy4 mutant background). GFP-OsCRY1 plants showed shorter hypocotyls under blue light condition. By the addition of nuclear localization signal (NLS) on the amino-terminal of GFP-OsCRY1, the enhanced inhibition of hypocotyl elongation was observed specifically under blue light. On contrast, the addition of nuclear export signal (NES) abolished the inhibitive effect of OsCRY1 overexpression. Thus, our results suggest that cryptochromes are imported into nucleus and function there, at least in the inhibition of hypocotyl elongation.

隐色素是调节植物发育的蓝光受体。虽然有报道称拟南芥隐色素1 （AtCRY1）定位于细胞核，但这一特征在各种植物物种中并不完全保守。本研究表明，水稻隐色素1的大部分分子定位于细胞核，但至少有一小部分定位于细胞质。为了确定隐色素的细胞内定位与功能之间的关系，我们构建了GFP-OsCRY1基因，并检测了其在转基因拟南芥（hy4突变体背景）中的活性。GFP-OsCRY1植株在蓝光条件下下胚轴变短。通过在GFP-OsCRY1的氨基端添加核定位信号（nuclear localization signal， NLS），在蓝光下观察到对下胚轴伸长的抑制增强。相反，核输出信号（NES）的加入消除了OsCRY1过表达的抑制作用。因此，我们的研究结果表明隐色素被输入细胞核并在那里发挥作用，至少在抑制下胚轴伸长方面。

项目成果

N.Matsumoto, T.Hirano, T.Iwasaki, N.Yamamoto: "Functional Analysis and Intracellular Localization of Rice Cryptochromes."Plant Physiology. 133(4). 1494-1503 (2003)

N.Matsumoto、T.Hirano、T.Iwasaki、N.Yamamoto：“水稻隐花色素的功能分析和细胞内定位”。植物生理学。

Functional analysis and intracellular localization of rice cryptoc hrome.

水稻隐色素的功能分析和细胞内定位。

• 发表时间: 2003
• 期刊: Plant Physiology 133
• 作者: Matsumoto;N. et al.
• 通讯作者: N. et al.

Functional analysis and intracellular localization of rice cryptochromes

• DOI: 10.1104/pp.103.025759
• 发表时间: 2003-12-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Matsumoto, N;Hirano, T;Yamamoto, N
• 通讯作者: Yamamoto, N

Automatic quantification of morphological traits via three-dimensional measurement of Arabidopsis

• DOI: 10.1111/j.1365-313x.2004.02042.x
• 发表时间: 2004-04-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Kaminuma, E;Heida, N;Toyoda, T
• 通讯作者: Toyoda, T