Regulation of Sympathetic Ganglion Development in Vitro

体外交感神经节发育的调节

• 批准号: 8819763
• 负责人: Karen Greif
• 金额: $ 19.86万
• 依托单位: Bryn Mawr College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8819763&HistoricalAwards=false
• 关键词: Regulation; Sympathetic; Ganglion; Development; Vitro

Dr. Greif will investigate factors which help regulate the development of the sympathetic nervous system, using the rat superior cervical ganglion (SCG) as a model system. The principal investigator will grow SCGs in a cell culture system, permitting a detailed analysis of how interactions between cells across synaptic junctions can alter the biochemical development of neurons. Dr. Greif will study how the levels of two proteins associated with synaptic vesicles, which package neurotransmitters, are changed when cells are depolarized electrically or when cells are grown in the presence of target tissue. Once the patterns of response are determined, she will examine how such factors can signal a cell to alter its production of a protein. She will focus on chemical signals found in cells known as "second messengers", which are important in regulating many cellular functions. SCGs will be treated with agents which directly change the level of second messengers. Dr. Greif will then study how well such treatments can mimic the effects of electrical depolarization or other factors. These studies will help us understand how cell-cell interactions can direct the normal development of the nervous system, and may provide important insights into factors responsible for disorders of neural development.

格雷夫博士将研究有助于调节 交感神经系统的发展，使用大鼠 上级颈神经节（SCG）作为模型系统。 的 首席研究员将在细胞培养系统中培养SCG， 从而详细分析细胞之间的相互作用 可以改变生物化学的发展 的神经元。 格雷夫博士将研究两种相关蛋白质的水平 与突触囊泡，包装神经递质， 当细胞被电去极化或当细胞被 在靶组织存在的情况下生长。 一旦这些模式 她将研究这些因素如何能够 向细胞发出信号以改变其蛋白质的产生。 她将 专注于细胞中发现的化学信号，称为“第二 信使”，这是重要的调节许多细胞 功能协调发展的 SCG将使用直接 改变第二信使的水平。 格雷夫博士将研究 这种治疗方法能模拟电流的效果 去极化或其他因素。 这些研究将帮助我们 了解细胞间的相互作用如何引导正常的 神经系统的发展，并可能提供重要的 对导致神经系统疾病的因素的深入了解 发展