Transplantation of the Sympathetic Ganglion in Parkinsonism

在1992-1993年科研资助计划(C)的资助下，我们对长期培养的移植物交感神经节的活性进行了研究。1992年，我们在6-羟基多巴胺损毁的大鼠身上检测了长期培养的交感神经节的活性。培养2周或4周的神经节在宿主脑内存活良好，表明6-OHDA损毁大鼠的异常行为有所恢复。相反，培养6周的神经节并未显示移植物的存活和行为的恢复。1993年，在神经节的培养过程中，我们在培养液中加入了IL-1来诱导神经生长因子。在IL-1条件培养液中培养的交感神经节显示出高浓度的神经生长因子(是对照组的7倍)，突起明显延长。将培养的交感神经节移植到白介素1条件培养液中，其存活率明显高于对照组，提示交感神经节在培养体系中处理4周可获得较高的移植物存活率，该方法可在临床上推广应用。

By the support of Grant-in-Aid for Scientific Research (C) from 1992-1993, we have studied viability of transplanted sympathetic ganglion which had been cultured for a long period. In 1992, viability of the long-term cultured sympathetic ganglion was examined in 6-OHDA lesioned rats. The ganglion cultured for 2, or 4 weeks well survived in the host brain and revealed recovery of abnormal behavior of the 6-OHDA lesioned rats. In contrast ganglion cultured for 6 weeks failed to show survival of the graft and recovery of the behavior. In 1993, during culture of the ganglion, we added Interleukin-1 to induce nerve growth factor in the cultured medium. The sympathetic ganglion cultured in the Interleukin-1-conditioned medium showed high concentration of nerve growth factor(seven times higher than the control) and extended neurite outgrowth. Transplantation of the cultured ganglion in the Interleukin-1-conditioned medium revealed higher viability than the control in the host brain.These results indicate the possibility of that the sympathetic ganglion can be treated in the culture system for 4 weeks to obtain high viability of the transplant, and this procedure can be clinically applied in the future.