Transplantation of stem cells from sympathetic ganglion into the brain Parkinson disease

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Stem cell has multipotent and self renewal ability, which is ideal source of donor for transplantation. We have studied transplantation of sympathetic ganglion into the brain with Parkinson disease, showing effect on parkinsonian symptoms such as bradykinesia and muscle rigidity. In the present research project we have applied transplantation of stem cells for Parkinson disease and examined effect of transplantation on parkinsonian symptoms. We also studied clinical research of sympathetic ganglion transplantation in the brain with Parkinson patients. Neural stem cells from nestin-GFP(green fluorescence protein) mouse were transplanted into the brain of 6-OHDA injected rat(an animal model of Parkinson disease). Transplanted stem cells well survived in the host brain. They increased cell numbers and extended their axons in the host brain. Surviving cells expressed TH imnmnoreactivity in the host brain. Survival rate in this experiment were 6-10%. Rotational scores after amphetamine administration were significantly reduced (77.3% on average) in the transplanted rats. Four patients undergoing thoracic sympathetic ganglia were carefully observed for long period, showing significant reduction in the percentage of time spent in the off phase. Piece of ganglion cultured in vitro revealed TH-, VMAT- and AADC- immunoreactivity.This research project clearly demonstrated the effects of stem cell transplantation on symptoms in animal model of Parkinson disease and parkinsonian patients.