Morphological study on the local circuits in the sympathetic ganglion To analyze a localized neuronal control system

1.大鼠脊髓的节前神经元在颈上神经节（SCG）的节后神经元周围形成篮状末梢神经丛。我们首先确定了神经节前神经元周围的篮状末梢神经丛的形成，这些神经元将轴突发送到下颌唾液腺。用WGA-TRITC逆行标记神经节后神经元，用抗calretinin抗体染色神经节前轴突。Carletinin是一种钙结合蛋白，存在于神经节前神经元中。证实了由分支轴突和多种静脉曲张组成的密集神经丛环绕支配颌下腺的神经节后神经元。电镜观察到SCG中有3种突触类型，即1)轴-树突突触，2)轴-树突棘突触和树突-树突突触。其次，用激光共聚焦显微镜观察神经递质在神经节前末梢的共存，确定了乙酰胆碱、GABA、NOS （NO）和calretinin。以囊泡乙酰胆碱转运蛋白作为胆碱能轴突的标记，GAD65作为gab能轴突，谷氨酸转运蛋白1、2、3作为谷氨酸能轴突，nNOS作为含NO轴突，研究神经递质及相关分子在SCG节前轴突中的共存情况，采用双抗体染色检测。乙酰胆碱- no - gaba -谷氨酸降低了它们的存在。已有报道称，GABA阳性神经元是位于交感颈神经节或交感干的类似SIF细胞的小或中大型神经元。我们重新研究了这个问题。我们发现，向SCG发送终端的gabargic神经元是位于中央管周围正中区域的神经节前神经元。用抗GAD65和顺行标记物质双染色法对星状神经节前轴突进行顺行染色，对星状神经节前纤维进行顺行染色。少

1.The preganglionic neurons in the rat spinal cord make a basket-like terminal plexus surrounding the postganglionic neurons in the superior cervical ganglion (SCG). We certained at first a formation of the basket-like termial plexus of the preganglionic neurons surrounding neurons sending their axons to the submandibular salivary gland. The postganlionic neurons were retrogradely labeled with WGA-TRITC, and the preganglionic axons were stained with anti-calretinin antibody. Carletinin is a Ca binding protein and coexists in the preganglionic neurons. It was confirmed that the dense plexus eonsiting of branching axons and many varicosities encircled the postganglionic neurons innervating the submandibular gland. With electronmicroscopy three types of synapses were observed in the SCG, that is, 1)axo-dendritic, 2)axo-dendritic spine, and dendro-dendritic synapse. Secondarily, the coexistence of neurotransmitters in the preganglionic terminals were investigated with a laser confocul micr … More oscope, and it was certained that acetylcholine, GABA, NOS (NO), and calretinin.2.Using vesicular acetylcholine transporter as a marker of cholinergic axon, GAD65 as GABAergic axon, vesicular glutamae transporter 1,2,3 as glutamatergic axon and nNOS as NO containing axon the coexicetence of neurotransmitters or relating molecules were studied in the preganglionic axons in the SCG was examined with double antibodies staining. In order to acetylcholine-NO-GABA-glutamate their existence decreased.3.It has been already reported that GABA positive neurons were a small or middle-large neurons resembling SIF cells located in the sympathetic cervical ganglia or sympathetic trunk. We reexamined this subject. We found that the gabargic neurons sending the terminals to the SCG were preganglionic neurons situated in the median regions surrounding the central canal. We also anterogradely stained the preganglionic axons in the stellate ganglion and SCG the preganglionic fibers using a double staining method with anti GAD65 and anterograde labeling substance. Less