Morphological study on the local circuits in the sympathetic ganglion To analyze a localized neuronal control system

1.大鼠脊髓的节前神经元在上级颈神经节（SCG）的节后神经元周围形成篮状终丛。我们首先确定了一个篮状终丛的神经节前神经元周围的神经元发送到下颌下腺的轴突的形成。用WGA-TRITC逆行标记节后神经元，用抗calretinin抗体染色节前轴突。Carletinin是一种钙结合蛋白，共存于节前神经元中。证实支配下颌下腺的节后神经元周围有由分支轴突组成的密集丛和许多静脉曲张。电镜下观察到3种类型的突触：1）轴-树突触，2）轴-树棘突触和树-树突触。用激光共聚焦显微镜观察神经递质在节前末梢的共存情况 关于我们 2.以囊泡乙酰胆碱转运体为胆碱能轴突标记物，GAD 65为GABA能轴突标记物，囊泡谷氨酸转运体1、2、3为谷氨酸能轴突标记物，nNOS为含NO轴突标记物，用双抗体染色法研究了SCG节前轴突内神经递质及相关分子的共存。3.已有报道GABA阳性神经元是位于颈交感神经节或交感干内的类似SIF细胞的小型或中大型神经元。我们重新审视了这个问题。我们发现，GABA能神经元发送终端的SCG位于中央管周围的正中区域的节前神经元。用抗GAD 65抗体和顺行标记物双重染色法对星状神经节的节前轴突和节前纤维进行了顺行染色。少

1.The preganglionic neurons in the rat spinal cord make a basket-like terminal plexus surrounding the postganglionic neurons in the superior cervical ganglion (SCG). We certained at first a formation of the basket-like termial plexus of the preganglionic neurons surrounding neurons sending their axons to the submandibular salivary gland. The postganlionic neurons were retrogradely labeled with WGA-TRITC, and the preganglionic axons were stained with anti-calretinin antibody. Carletinin is a Ca binding protein and coexists in the preganglionic neurons. It was confirmed that the dense plexus eonsiting of branching axons and many varicosities encircled the postganglionic neurons innervating the submandibular gland. With electronmicroscopy three types of synapses were observed in the SCG, that is, 1)axo-dendritic, 2)axo-dendritic spine, and dendro-dendritic synapse. Secondarily, the coexistence of neurotransmitters in the preganglionic terminals were investigated with a laser confocul micr … More oscope, and it was certained that acetylcholine, GABA, NOS (NO), and calretinin.2.Using vesicular acetylcholine transporter as a marker of cholinergic axon, GAD65 as GABAergic axon, vesicular glutamae transporter 1,2,3 as glutamatergic axon and nNOS as NO containing axon the coexicetence of neurotransmitters or relating molecules were studied in the preganglionic axons in the SCG was examined with double antibodies staining. In order to acetylcholine-NO-GABA-glutamate their existence decreased.3.It has been already reported that GABA positive neurons were a small or middle-large neurons resembling SIF cells located in the sympathetic cervical ganglia or sympathetic trunk. We reexamined this subject. We found that the gabargic neurons sending the terminals to the SCG were preganglionic neurons situated in the median regions surrounding the central canal. We also anterogradely stained the preganglionic axons in the stellate ganglion and SCG the preganglionic fibers using a double staining method with anti GAD65 and anterograde labeling substance. Less