Morphological study on the local circuits in the sympathetic ganglion To analyze a localized neuronal control system

1.大鼠脊髓节前神经元围绕颈上神经节节后神经元形成篮状终末神经丛。我们首先确定了神经节前神经元周围的篮状终末神经丛的形成，这些神经元将轴突发送到颌下唾液腺。用WGA-TRITC逆行标记节后神经元，用抗Calretinin抗体对节前轴突进行染色。Carletinin是一种钙结合蛋白，共存于节前神经元。结果证实，致密的分支轴突和许多曲张的神经丛环绕着支配颌下腺的节后神经元。用电子显微镜观察到SCG内有三种类型的突触，即1)轴-树突触，2)轴-树突触，以及树-树突触。其次，用激光共聚焦显微镜…研究了神经节前终末内神经递质的共存2.以囊泡型乙酰胆碱转运蛋白作为胆碱能轴突的标记物，GAD65为GABA能轴突，囊泡型谷氨酸转运蛋白1，2，3为谷氨酸能轴突，nNOS为含NO轴突，用双抗体染色方法研究了SCG节前轴突内神经递质或相关分子的共存情况。为了乙酰胆碱-NO-GABA-谷氨酸，它们的存在减少了。3.已有报道称，GABA阳性神经元是一种类似于SIF细胞的中、小型神经元，位于交感神经节或交感干内。我们重新审视了这个主题。我们发现，将终末送到SCG的辉长神经元是位于中央管周围正中区域的节前神经元。我们还用抗GAD65和顺行标记物质的双重染色方法对星状神经节节前轴突和节前纤维进行顺行染色。较少

1.The preganglionic neurons in the rat spinal cord make a basket-like terminal plexus surrounding the postganglionic neurons in the superior cervical ganglion (SCG). We certained at first a formation of the basket-like termial plexus of the preganglionic neurons surrounding neurons sending their axons to the submandibular salivary gland. The postganlionic neurons were retrogradely labeled with WGA-TRITC, and the preganglionic axons were stained with anti-calretinin antibody. Carletinin is a Ca binding protein and coexists in the preganglionic neurons. It was confirmed that the dense plexus eonsiting of branching axons and many varicosities encircled the postganglionic neurons innervating the submandibular gland. With electronmicroscopy three types of synapses were observed in the SCG, that is, 1)axo-dendritic, 2)axo-dendritic spine, and dendro-dendritic synapse. Secondarily, the coexistence of neurotransmitters in the preganglionic terminals were investigated with a laser confocul micr … More oscope, and it was certained that acetylcholine, GABA, NOS (NO), and calretinin.2.Using vesicular acetylcholine transporter as a marker of cholinergic axon, GAD65 as GABAergic axon, vesicular glutamae transporter 1,2,3 as glutamatergic axon and nNOS as NO containing axon the coexicetence of neurotransmitters or relating molecules were studied in the preganglionic axons in the SCG was examined with double antibodies staining. In order to acetylcholine-NO-GABA-glutamate their existence decreased.3.It has been already reported that GABA positive neurons were a small or middle-large neurons resembling SIF cells located in the sympathetic cervical ganglia or sympathetic trunk. We reexamined this subject. We found that the gabargic neurons sending the terminals to the SCG were preganglionic neurons situated in the median regions surrounding the central canal. We also anterogradely stained the preganglionic axons in the stellate ganglion and SCG the preganglionic fibers using a double staining method with anti GAD65 and anterograde labeling substance. Less