RUI: Control of Membrane Development

RUI：膜发育的控制

• 批准号: 8820092
• 负责人: Anthony Andreoli
• 金额: $ 11.8万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820092&HistoricalAwards=false
• 关键词: RUI; Control; Membrane; Development

Sequential changes in gene expression control differentiation in Bacillus subtilis during sporulation. Little information exists on the gene products of early sporulation, when rapid assembly of unique, intracellular, sporulation-specific membranes occurs, i.e. asymmetric spore septum and engulfment membranes, to establish the intracellular forespore. Dr. Andreoli has demonstrated 8 unique membrane proteins in forespore membranes and isolated genes for 4 of the 8 proteins (S1, S2, E3 and E4) by screening with specific IgG B. subtilis genomic library. Restriction maps for the cloned S1 E3, and E4 inserts were constructed. The S1, S2, E3 and E4 genes will be isolated by subcloning into lambda ZAP to locate the relevant genes in the inserts recovered from the genomic library and the genes will be mapped in the B. subtilis chromosome. The results should tell whether each of the membrane protein genes map at known spo loci, or others. %%% Current understanding of B. Subtilis molecular genetics makes this cell system ideal for studies of the control of sporulation. The early stages of this dramatic developmental change involve the rapid development of unique, intracellular, sporulation- specific membranes. This work will allow the identification of and role for some of those proteins.

基因表达的顺序变化控制着 芽孢形成过程中的枯草芽孢杆菌。 信息很少 在早期孢子形成的基因产物上，当快速组装 出现独特的胞内孢子形成特异性膜， 即不对称的孢子隔膜和吞噬膜， 建立胞内前孢子。 安德烈奥利博士 在前孢子膜中发现了8种独特的膜蛋白 并分离8种蛋白质中的4种（S1、S2、E3和E4）的基因， 用特异性IgG B筛查。枯草杆菌基因组文库 克隆的S1、E3和E4插入片段的限制性酶切图谱被克隆。 构建了将通过以下方法分离S1、S2、E3和E4基因： 亚克隆入λ ZAP以定位相关基因， 从基因组文库中回收的插入物， 映射在B中。枯草染色体结果应该告诉我们 无论每个膜蛋白基因是否定位于已知的SPO基因座， 或其他人。 %%% 当前对B的理解。枯草杆菌分子遗传学使 该细胞系统非常适合研究控制孢子形成。 这种戏剧性的发展变化的早期阶段包括 独特的细胞内孢子形成的快速发展， 特定的膜。这项工作将有助于确定 以及其中一些蛋白质的作用。