RUI: Acquisition of a Preparative Ultracentriguge: A Multi-User Request

RUI：获取制备型超速离心机：多用户请求

• 批准号: 8820311
• 负责人: Paulette Royt
• 金额: $ 3万
• 依托单位: George Mason University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820311&HistoricalAwards=false
• 关键词: RUI; Acquisition; Preparative; Ultracentriguge; Multi

A preparative ultracentrifuge and two rotors will be placed in the Biology Department of George Mason University. The high speed capability of the ultracentrifuge and the large volume capacity of the rotors are needed 1) for the continuation of research of at least five professors in the Biology Department of this University, and 2) in the laboratory instruction undergraduate students in this Department. By facilitating methodologies of cell fractionation, the ultracentrifuge will impact the following research projects: the study of iron transport into the bacterium Pseudomonas aeruginosa, the localization of the electron transport components of an iron- reducing bacterium, the isolation of membranes involved in extracellular polysaccharide synthesis and modification in red algae, the role of thymic growth factors on human breast cancer cells growing in culture, and the role of stress proteins in the acquisition of stress tolerance. As well, the instrument will be used to train students in the research laboratory of each professor. It will also be used to teach students in the classroom laboratory the basic techniques of cell fractionation and virus isolation. Students enrolled in Biological Ultrastructure, Experiments in Microbiology, Techniques in Virology, and Advanced Techniques in Molecular Biology will use the ultracentrifuge in laboratory experiments.//

一个准备超级电容器和两个转子将被放置在 乔治梅森大学生物系。 高 超高速、大体积的高速性能 转子的容量是需要的1）为继续 生物系至少五位教授的研究 这所大学，和2）在实验室教学 本系本科生。 通过促进 细胞分级分离的方法，超浓缩将 影响以下研究项目：铁的研究 运输到细菌铜绿假单胞菌， 铁的电子传递成分的定位- 还原细菌，分离膜参与 红细胞胞外多糖的合成与修饰 藻类，胸腺生长因子对人乳腺癌的作用 细胞在培养中的生长，以及应激蛋白在 获得抗逆能力。 此外，该仪器将 用于在每个研究实验室培训学生 教授 它也将被用来教学生在 课堂实验室细胞分级分离的基本技术 和病毒分离。 生物学专业学生 超微结构，微生物学实验， 病毒学和分子生物学高级技术将使用 实验室实验中的超浓缩物。