Acquisition of a Preparative Ultracentrifuge

化学研究仪器和设施计划(CRIF)的这一奖项将帮助纽约州立大学石溪分校化学系获得预备性超速离心机。该设备增强的化学领域包括：(1)用于磁共振成像的新的磁共振造影剂的设计和表征，(2)辅酶A和棕榈酰辅酶A类似物的合成，(3)核糖体蛋白L9和E3/E1P结构域的折叠，(4)蛋白质表面环和铰链在磷酸丙糖异构酶和界面酶胆固醇氧化酶催化的反应中的作用，(5)受精素-β在其受体中的结合位置，(6)作为天冬氨酸和金属蛋白酶抑制剂的多官能有机硅烷的合成，(7)界面酶磷脂酶D的研究；(8)Enoyl-辅酶A水合酶和Enoyl-ACP还原酶催化反应中导致催化的分子作用力的光谱分析。该仪器还将用于两个高级本科实验室。超速离心法是一种主要用于帮助分离和纯化蛋白质的技术。化学家在生物有机化学领域进行尖端研究的能力要求获得最先进的制备超速离心机。

This award from the Chemistry Research Instrumentation and Facilities Program (CRIF) will assist the Department of Chemistry of SUNY at Stony Brook in acquiring a preparative ultracentrifuge. The areas of chemistry enhanced by the equipment include: (1) the design and characterization of new magnetic contrast agents for magnetic resonance imaging, (2) the synthesis of coenzyme A and palmitoyl-coenzyme A analogs, (3) the folding of the ribosomal protein L9 and of the E3/E1P domain, (4) the role of protein surface loops and hinges in the reactions catalyzed by triosephosphate isomerase and the interfacial enzyme cholesterol oxidase, (5) the binding site for fertilin-beta in its receptor, (6) the synthesis of polyfunctional organosilanes as inhibitors of aspartic and metalloproteases, (7) the study of the interfacial enzyme phospholipase D and (8) the spectroscopic analysis of the molecular forces leading to catalysis in the reactions catalyzed by enoyl-coenzyme A hydratase and enoyl-acp reductase. The instrument will also be used in two advanced undergraduate laboratories. Ultracentrifugation is a technique used mainly to aid in the isolation and purification of proteins. The capability of chemists to carry out cutting edge research in bioorganic chemistry demands access to state-of-the-art preparative ultracentrifuges.