Acquisition of Preparative HPLC System

购置制备型 HPLC 系统

• 批准号: 8904468
• 负责人: Robert Harris
• 金额: $ 3.04万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904468&HistoricalAwards=false
• 关键词: Acquisition; Preparative; HPLC; System

This is a proposal to acquire a Preparative HPLC System to be used primarily for the purification of synthetic peptides. The science sections detailed in this proposal describe the role of peptide synthesis in each investigator's program. The ability to purify large (mg) quantities of the synthesized peptides by preparative HPLC is essential for continued (1) Studies on processing mechanisms of peptide-hormones and conformation of pro-hormone precursor proteins (2) Studies on the structure of hepatitis B viral antigens (3) and (4) Studies on the mechanism os nonenzymatic de-amidation of Asn and Gln residues in peptides and proteins (5) Studies on the effects of snake venoms on hemostasis and the development of synthetic anticoagulant peptides (6) Studies on the mitogenic activity of synthetic growth hormone fragments (7) Studies on mitogenic factors for cultured glial cells (8) Studies on the modification of alkaline phosphatase in vitro and in vivo.

这是一项购买制备型 HPLC 系统的提案，主要用于合成肽的纯化。 该提案中详细的科学部分描述了肽合成在每个研究者的计划中的作用。 通过制备型 HPLC 纯化大量 (mg) 合成肽的能力对于继续 (1) 研究肽激素的加工机制和激素原前体蛋白的构象 (2) 研究乙型肝炎病毒抗原的结构 (3) 和 (4) 研究肽和肽中 Asn 和 Gln 残基的非酶促脱酰胺机制至关重要。 （5）蛇毒止血作用研究及合成抗凝肽的开发（6）合成生长激素片段促有丝分裂活性研究（7）培养胶质细胞促有丝分裂因子研究（8）碱性磷酸酶体内外修饰研究。