Joel 1200EX/TN-8500 Electron Microscope System

请拨经费购买JEOL JEM 1200EX/TN-8500，这是一种能够连续重建的最先进的透射电子显微镜和图像分析系统(Tracor Northern)。用户组的所有成员都在神经系统中进行不同的准备工作，但都有一个共同的需求，即需要一种具有复杂图像处理能力的电子显微镜，包括3-D序列重建。其中5名使用者的项目涉及将辣根过氧化物酶注射到生理识别的神经元中，并随后对识别的神经元的突触电路进行超微结构分析。正在研究的系统包括视网膜、中央视觉通路、心脏和脊髓的神经控制。第六位用户正在研究中枢神经系统中的毛细血管。目前使用的电子显微镜陈旧(10年和15年)，持续供应高度不确定，不能达到现代神经解剖学研究所需的分析水平。

Funds are requested for a JEOL JEM 1200EX/TN-8500, which is a state-of-art transmission electron microscope and image analysis system (Tracor Northern) that is capable of serial reconstruction. All members of the user group work on different preparations in the nervous system, but have a common need for the availability of an electron microscope with sophisticated image processing capabilities, including 3-D serial reconstruction. The projects of 5 of the users involve the injection of horseradish peroxidase into physiologically identified neurons and the subsequent ultrastructural analysis of the synaptic circuitry of the identified neurons. The systems under investigation include the retina, central visual pathways, neural control of the heart and the spinal cord. The sixth user is studying capillaries in the central nervous system. The currently used electron microscopes are old (10 and 15 years), of highly uncertain continued availability, and not capable of the levels of analysis required for modern neuroanatomical studies.