Joel 1200EX/TN-8500 Electron Microscope System

请求为一架JEOL JEM 1200EX/TN-8500型飞机提供资金， 最先进透射电子显微镜和图像分析 系统（Tracor北方），能够串行 重建 用户组的所有成员都在不同的 在神经系统中的制剂，但有一个共同的需要， 电子显微镜的可用性， 图像处理能力，包括3D串行 重建 5个用户的项目涉及 将辣根过氧化物酶注射到生理 识别神经元和随后的超微结构分析， 神经元的突触回路 的系统 正在研究的包括视网膜，中央视觉通路， 心脏和脊髓的神经控制。 第六用户 正在研究中枢神经系统中的毛细血管 的 目前使用的电子显微镜是旧的（10年和15年）， 高度不确定的持续可用性， 现代神经解剖学研究所需的分析水平。

Funds are requested for a JEOL JEM 1200EX/TN-8500, which is a state-of-art transmission electron microscope and image analysis system (Tracor Northern) that is capable of serial reconstruction. All members of the user group work on different preparations in the nervous system, but have a common need for the availability of an electron microscope with sophisticated image processing capabilities, including 3-D serial reconstruction. The projects of 5 of the users involve the injection of horseradish peroxidase into physiologically identified neurons and the subsequent ultrastructural analysis of the synaptic circuitry of the identified neurons. The systems under investigation include the retina, central visual pathways, neural control of the heart and the spinal cord. The sixth user is studying capillaries in the central nervous system. The currently used electron microscopes are old (10 and 15 years), of highly uncertain continued availability, and not capable of the levels of analysis required for modern neuroanatomical studies.