JEOL JEM 1200EX TEMSCAN ELECTRON MICROSCOPE

JEOL JEM 1200EX TEMSCAN 电子显微镜

• 批准号: 3519274
• 负责人: ARTHUR VEIS
• 金额: $ 12.5万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-02-01 至 1986-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519274
• 关键词: cell membrane; dental materials; electron microscopy; electron probe spectrometry; morphology; normal ossification; scanning electron microscopy

This application is for an electron microscope to replace outmoded equipment. The instrument of choice is the JEOL, JEM 1200EX Analytical TEMSCAN with high resolution transmission (TEM), scanning (SEM) and scanning transmission (STEM) modes, eucentric goniometer and facilities for selected area x-ray probe microanalysis. This instrument will be utilized by a multi-user core group. The projects to be undertaken require the full versatility of the microscope. 1) Cell morphology and surface structure. Macrophages and mesenchymal cells, their surface membranes, and their interactions with each other and with specific targets will be examined in relation to their function in would repair, inflammation and cellular hemeostasis. Fibroblast aging in vivo and in vitro, and the distribution of various surface molecules will be examined using specific labelling procedures. 2) Studies of collagen structure and mode of assembly. The intracellular site(s) of collagen triple helix formation will be studied. Collagen mRNA containing nascent collagen chains will be examined in the intact state and following several specific enzyme treatments. The structure of collagen fibrils will be studied, by chemically modifying exposed amino- or carboxyl-groups within fibrils, and locating modified groups from the changes in the SLS banding patterns. Specific end regions of collagen molecules will be localized in fibrils using labelled antibody techniques. The structure of basement membrane components will also be studied. 3) Bone and dentin mineralization. The location of epitaxial factors in mineralizing tissues will be examined to determine their relationship to the mineralization front and intracellular route of secretion. Selected area x-ray probe microanalysis will also be used to determine the location of collagen-bound phosphoproteins with high Ca++ affinity within mineralizing tissues. 4) Dental Materials. Studies on the corrosion of dental alloys, on retreival analysis of dental amalgams and on the development of Ti alloys and porcelains will be carried out. Stress induction at porcelain-metal interfaces will also be considered. The EDAX equipment is a prime requirement for these studies. A specific plan for control of usage, user fees, instrumental maintenance and other administrative aspects is presented.

此应用程序是电子显微镜，以取代过时的 设备. 选择的仪器是JEOL，JEM 1200 EX分析仪 具有高分辨率透射（TEM）、扫描（SEM）和 扫描传输（STEM）模式、同心测角仪和设施， 选区X射线探针微区分析。 将利用这一工具 一个多用户核心组。 要开展的项目需要充分 显微镜的多功能性。 1)细胞形态和表面结构。 巨噬细胞和间充质细胞，它们的表面膜， 相互之间以及与特定目标之间的相互作用将在 与它们在伤口修复、炎症和细胞 血液平衡。 成纤维细胞在体内和体外的老化和分布 各种表面分子将使用特定的标记进行检查 程序. 2)胶原结构和组装模式的研究。 的 将研究胶原三螺旋形成细胞内位点。 含有新生胶原蛋白链的胶原蛋白mRNA将在 完整状态和几种特定的酶处理。 的 胶原纤维的结构将被研究，通过化学修饰 原纤维内暴露的氨基或羧基， 从SLS带型的变化组。 特定末端区域 胶原蛋白分子将被定位在纤维使用标记抗体 技术. 基底膜组件的结构也将被 研究了 3)骨和牙本质矿化。 外延的位置 矿化组织中的因素将被检查，以确定其 与矿化前沿和细胞内途径的关系 分泌物 选定区域的X射线探针显微分析也将用于 确定高Ca++的胶原结合磷蛋白的位置 在矿化组织中的亲和力。 4)牙科材料。 的研究 牙科合金的腐蚀，牙科汞合金的回收分析， 开展钛合金和陶瓷的开发。 应力 还将考虑在陶瓷-金属界面处的感应。 EDAX 设备是这些研究的首要要求。 的具体方案 控制使用、用户费用、仪器维护和其他 行政方面提出。