JEOL JEM 1200EX TEMSCAN ELECTRON MICROSCOPE

这项应用是为了让电子显微镜取代过时的 设备。选择的仪器是JEOL，JEM 1200EX Analytical TEMSCAN具有高分辨率透射(TEM)、扫描(SEM)和 扫描传输(STEM)模式、恒心测角仪和用于 选区X射线探头微区分析。这台仪器将被利用 由多用户核心组提供。将要进行的项目需要充分的 显微镜的多功能性。1)细胞形态和表面结构。 巨噬细胞和间充质细胞及其表面膜和它们的 中将研究彼此之间的交互以及与特定目标的交互 它们与Will修复、炎症和细胞功能的关系 止血作用。成纤维细胞在体内和体外的老化及其分布 不同的表面分子将使用特定的标记进行检查 程序。2)胶原结构和组装方式的研究。这个 将研究胶原蛋白三螺旋形成的胞内位点(S)。 含有新生胶原链的胶原蛋白mRNA将在 完整状态，并在几种特定的酶处理之后。这个 将通过化学修饰来研究胶原纤维的结构 纤维内暴露的氨基或羧基，以及定位修饰 从SLS带型的变化中得出结论。特定端部区域 的胶原蛋白分子将使用标记抗体定位在纤维中 技巧。基底膜组件的结构也将是 学习。3)骨和牙本质矿化。外延的位置 将检查矿化组织中的因素以确定其 与成矿前沿和胞内路径的关系 分泌物。选定区域的X射线探头微量分析也将用于 测定高Ca++的胶原结合磷蛋白的位置 矿化组织内的亲和力。4)牙科材料。关于中国石油天然气集团公司 牙科合金的腐蚀，对牙科银汞合金修复分析的影响等 发展钛合金和钛瓷器。压力 瓷器-金属界面的感应也将被考虑。EDAX 设备是这些研究的首要要求。一个具体的计划 控制使用、用户费用、仪器维护和其他 介绍了管理方面的内容。

This application is for an electron microscope to replace outmoded equipment. The instrument of choice is the JEOL, JEM 1200EX Analytical TEMSCAN with high resolution transmission (TEM), scanning (SEM) and scanning transmission (STEM) modes, eucentric goniometer and facilities for selected area x-ray probe microanalysis. This instrument will be utilized by a multi-user core group. The projects to be undertaken require the full versatility of the microscope. 1) Cell morphology and surface structure. Macrophages and mesenchymal cells, their surface membranes, and their interactions with each other and with specific targets will be examined in relation to their function in would repair, inflammation and cellular hemeostasis. Fibroblast aging in vivo and in vitro, and the distribution of various surface molecules will be examined using specific labelling procedures. 2) Studies of collagen structure and mode of assembly. The intracellular site(s) of collagen triple helix formation will be studied. Collagen mRNA containing nascent collagen chains will be examined in the intact state and following several specific enzyme treatments. The structure of collagen fibrils will be studied, by chemically modifying exposed amino- or carboxyl-groups within fibrils, and locating modified groups from the changes in the SLS banding patterns. Specific end regions of collagen molecules will be localized in fibrils using labelled antibody techniques. The structure of basement membrane components will also be studied. 3) Bone and dentin mineralization. The location of epitaxial factors in mineralizing tissues will be examined to determine their relationship to the mineralization front and intracellular route of secretion. Selected area x-ray probe microanalysis will also be used to determine the location of collagen-bound phosphoproteins with high Ca++ affinity within mineralizing tissues. 4) Dental Materials. Studies on the corrosion of dental alloys, on retreival analysis of dental amalgams and on the development of Ti alloys and porcelains will be carried out. Stress induction at porcelain-metal interfaces will also be considered. The EDAX equipment is a prime requirement for these studies. A specific plan for control of usage, user fees, instrumental maintenance and other administrative aspects is presented.