Motility and Chemotaxis in Spirochetes

螺旋体的运动性和趋化性

• 批准号: 8913528
• 负责人: Everett Greenberg
• 金额: $ 29.5万
• 依托单位: University of Iowa
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-15 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8913528&HistoricalAwards=false
• 关键词: Motility; Chemotaxis; Spirochetes

Unlike well studied bacteria such as Eschericia coli, the membrane potential of Spirochaeta aurantia is involved in chemotaxis. Furthermore the flagellar filaments of the spirochete periplasmic flagella are relatively complex when compared with the exoflagella of other bacteria. The goal of the proposed research is to understand the molecular basis of the mechanisms of motility and chemotaxis unique to spirochetes. Genes required for motility will be cloned and analyzed. A considerable effort toward developing a gene transfer system for this organisms will be mounted. The immediate use of such a system will be in studies of spirochete motility and chemotaxis. Techniques for studying ionic channels in the membranes of S. aurantia will be developed with an ultimate goal of identifying chemoeffector-regulated channels. Spirochetes are motile bacteria that have periplasmic flagella rather than exoflagella. Cells exhibit smooth swimming, reversals of cell orientation and flexing. At least some spirochetes also can "crawl" on solid surfaces. The peculiar mechanism of spirochete motility is important to the success of these organisms in the viscous environments in which they trive. The proposed research will employ Spirochaeta aurantia as a model in studies of motility and chemotaxis of spirochetes and in the development of techniques generally useful for studies of spirochetes. The results of this research should provide new information concerning the relationship of the unique spirochete motility system to their behavior.

与研究得很好的细菌（如土栖性大肠杆菌）不同， 橙色螺旋体的膜电位参与 趋化性 此外， 螺旋体周质鞭毛相对复杂， 与其他细菌的外鞭毛相比。 的目标 建议的研究是了解的分子基础， 螺旋体特有的运动和趋化机制。 将克隆和分析运动所需的基因。 一 为了开发用于治疗癌症的基因转移系统， 这些生物将被安装。 立即使用这种 该系统将用于螺旋体运动和趋化性的研究。 本文介绍了研究S. aurantia的最终目标是识别 化学效应调节通道。 螺旋体是具有周质鞭毛的能动细菌 而不是外鞭毛 细胞表现出平滑的游动， 细胞取向和弯曲的逆转。 至少一些 螺旋体也能在固体表面上“爬行”。 特有的 螺旋体运动的机制对于成功地 这些有机体在粘性环境中生存。 拟议的研究将采用橙色螺旋体作为模型 在螺旋体的运动性和趋化性的研究中， 发展对研究普遍有用的技术 螺旋体 这项研究的结果将提供新的 关于独特的螺旋体 运动系统对其行为的影响。