Pancreatic Hormones and Their Functions in Agnathans and Teleost Fishes

无颌类和硬骨鱼中的胰腺激素及其功能

• 批准号: 8915935
• 负责人: Erika Plisetskaya
• 金额: $ 45.91万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8915935&HistoricalAwards=false
• 关键词: Pancreatic; Hormones; Their; Functions; Agnathans

Fish have served as objects of experimental research for several centuries, however their role as an experimental model, which can substitute for conventional laboratory warmblooded animals has been enhanced particularly in recent times. Representative agnathans (lamprey or hagfish) and teleost fish (salmon, trout, catfish) are to be used in studies of the regulation of metabolism and growth by pancreatic hormones, their interaction with each other and with growth hormone and insulin-like growth factor. Specific objectives of the project are: 1) to develop new homologous radioimmunoassays or enzyme- linked immunoassays for measurement of the islet hormones in lampreys; 2) to continue an assessment of the profiles of pancreatic hormones in plasma and endocrine pancreas of salmon, trout and lamprey at different stages of their life cycles and kept under different feeding regimes; 3) to determine the levels of pancreatic hormones in the blood outflow from the pancreatic islets (major producing sites) and from the liver, (important target and main place for destruction of the pancreatic hormones); to evaluate the differences in the hormonal contents in fasting and feeding animals with respect to these patterns; 4) to examine whether the cells from different parts of liver, encountering different levels of hormones are metabolically differentiated and to employ isolated hepatocytes as an experimental in vitro system for investigation of the effects of glucagon-family peptides, which regulate energy stores; 5) to study specific binding of the major anabolic hormone, insulin to its target, the liver, at different developmental stages and under different feeding regimes; 6) to investigate whether the unusual abundance of "big" somatostatins in the endocrine pancreas of lamprey and salmon has any particular physiological significance. The expected results will contribute to better understanding of hormonal regulation of nutrition and growth as well as extend our knowledge of functional evolution of pancreatic hormones in vertebrates.

鱼类已经作为实验研究的对象， 几个世纪以来，它们作为实验模型的作用， 传统实验室温血动物的替代品， 尤其是在最近一段时间。 代表性无颌类 （七鳃鳗或八目鳗）和硬骨鱼（鲑鱼，鳟鱼，鲶鱼）是为了 用于研究代谢和生长的调节， 胰腺激素，它们之间的相互作用以及与生长 激素和胰岛素样生长因子。 的具体目标 项目是：1）开发新的同源放射免疫测定或酶- 用免疫联用法测定七鳃鳗的胰岛激素; 2)继续评估患者的胰腺激素水平 血浆和内分泌胰腺的鲑鱼，鳟鱼和七鳃鳗在不同的 生活史的各个阶段，并保持在不同的喂养制度; 3)来测定流出血液中的胰腺激素水平 从胰岛（主要产生部位）和从肝脏， （胰腺的重要靶点和主要破坏部位 激素）;评估激素含量的差异 根据这些模式禁食和喂养动物; 4） 检查来自肝脏不同部位的细胞， 不同水平的激素在代谢上是不同的， 使用分离的肝细胞作为体外实验系统， 研究胰高血糖素家族肽的作用， 调节能量储存; 5）研究主要的特异性结合 合成代谢激素，胰岛素到其目标，肝脏，在不同的 发育阶段和不同的喂养制度; 6）， 调查是否异常丰富的“大”生长抑素在 七鳃鳗和鲑鱼的内分泌胰腺 生理意义 预期成果将有助于 更好地了解营养和生长的激素调节， 并扩展我们对胰腺功能进化的认识， 脊椎动物体内的激素