The ACC Synthase Gene

ACC合成酶的诱导机制， 植物乙烯生物合成途径中的酶，通过各种 对植物激素和环境刺激的了解很少。 最近在Theologis博士的实验室获得的实验证据， 与ACC合酶mRNA互补的DNA序列来自 西葫芦表明，酶诱导剂，如IAA，伤害李 离子在转录或转录后水平起作用。 ACC合酶基因的结构与调控研究 在西葫芦和番茄中， 调节它们的生化机制的细节 表达的各种激素和压力条件，以及 在调节植物衰老中的作用。具体目标 1）隔离和结构 ACC的互补序列和基因组序列的表征 西葫芦和番茄合成酶mRNA，2）发育 生长期间ACC合酶基因表达的调节，3） 确定这种顺式作用的DNA调控元件， 参与乙烯和生长素调控的ACC合成酶基因 4）ACC合酶基因表达的调节 在体内和分离的细胞核中对各种激素的反应 和环境胁迫条件下，5）抑制乙烯 ACC诱导果实成熟和厌氧诱导上位性 合成酶反义构建体。 %%% 乙烯是最简单的有机分子之一， 生物活性它被认为是水果成熟 已知调节植物生长许多方面激素， 发育和衰老。ACC合成酶是细胞内的关键酶， 乙烯合成途径。

The mechanism of induction of ACC synthase, the key regulatory enzyme in the ethylene biosynthetic pathway in plants, by various plant hormones and environmental stimuli is poorly understood. Recent experimental evidence obtained in Dr. Theologis' lab with DNA sequences complementary to the ACC synthase mRNA from zucchini indicate that enzyme inducers such as IAA, wounding Li ions act at the transcriptional or posttranscriptional level. Studies on the structure and regulation of ACC synthase gene(s) in zucchini and tomato are proposed to gain insight into the details of the biochemical mechanism that regulates their expression by various hormones and stress conditions as well as on their role in regulating plant senescence. The specific aims of this proposal are: 1) Isolation and structural characterization of complementary and genomic sequences to ACC synthase mRNA from zucchini and tomato, 2) Developmental regulation of ACC synthase gene expression during growth, 3) Determination of this cis acting DNA regulatory elements that are involved in ethylene and auxin regulated ACC synthase gene expression, 4) Regulation of expression of ACC synthase gene(s) in vivo and in isolated nuclei in response to various hormones and environmental stress conditions, 5) Inhibition of ethylene induced fruit ripening and anaerobiosis-induced epinasty with ACC synthase antisense constructs. %%% Ethylene is one of the simplest organic molecules with biological activity. It is considered to be the fruit ripening hormone known to regulate many aspects of plant growth, development and senescence. ACC synthase is the key enzyme in the synthetic pathway of ethylene.