The ACC Synthase Genes and Polypeptides

ACC合酶基因和多肽

• 批准号: 0211921
• 负责人: Athanasios Theologis
• 金额: $ 49.57万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211921&HistoricalAwards=false
• 关键词: ACC; Synthase; Genes; Polypeptides

Ethylene is an unique plant signaling molecule. The hydrocarbon gas, known as the fruit ripening hormone, controls many aspects of plant growth and development. It is biosynthesized from methionine via a three-step enzymatic process: SAM ACC ACC Methionine SAM ACC C2H4. Synthase synthase oxidaseIts synthesis is highly regulated. Experimental evidence accumulated over the last 10 years indicates that one of the key enzymes in the ethylene biosynthetic pathway, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS), is encoded by a highly divergent multigene family in various mono- and dicotyledonous plants. The completion of the Arabidopsis genome sequence reveals the presence of 10 putative ACS genes (ACS2, 4-12) and two pseudogenes (ACS1, ACS3). Expression studies reveal that the family members are differentially regulated by various inducers and developmental stages Studies on the regulation of the ACS genes in Arabidopsis are proposed here to gain insight into the details of the signaling machinery involved primarily in the regulation of the ACS gene by auxin and ethylene. Further studies are proposed to obtain knowledge regarding the structure and the biochemical properties of the ACS isoenzymes. The proposed molecular and biochemical experimental approaches attempt to answer the following question: why are there 10 ACC synthase isoenzymes in Arabidopsis for the synthesis of the ethylene precursor ACC? While all of them have the same biochemical function, the question arises whether they have distinct or redundant biological function. The findings have the potential to allow a fundamental understanding of the role of multigene families in plants, a hallmark finding of the Arabidopsis Genome Sequencing project.

乙烯是一种独特的植物信号分子。 碳氢化合物气体，被称为水果成熟激素，控制着植物生长和发育的许多方面。 它是由蛋氨酸通过三步酶促过程生物合成的： SAM ACC ACC甲醇SAM ACC C2 H4。 合酶 合酶 氧化酶其合成受到高度调节。 在过去的10年中积累的实验证据表明，乙烯生物合成途径中的关键酶之一，1-氨基环丙烷-1-羧酸（ACC）合酶（ACS），是由一个高度分化的多基因家族在各种单子叶和双子叶植物的编码。 拟南芥基因组序列的完成揭示了10个推定的ACS基因（ACS 2，4-12）和两个假基因（ACS 1，ACS 3）的存在。 表达的研究表明，家庭成员的差异调节不同的诱导剂和发育阶段的研究ACS基因的调节拟南芥在这里提出了深入了解的细节，主要参与ACS基因的调节生长素和乙烯的信号机制。 进一步的研究建议获得有关ACS同工酶的结构和生化特性的知识。 所提出的分子和生物化学实验方法试图回答以下问题：为什么有10个ACC合成酶同工酶在拟南芥中的乙烯前体ACC的合成？ 虽然它们都具有相同的生物化学功能，但问题是它们是否具有独特或冗余的生物学功能。 这些发现有可能使人们对植物中多基因家族的作用有一个基本的了解，这是拟南芥基因组测序项目的一个标志性发现。