The ACC Synthase Genes and Polypeptides

ACC合酶基因和多肽

• 批准号: 9982895
• 负责人: Athanasios Theologis
• 金额: $ 12.92万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9982895&HistoricalAwards=false
• 关键词: ACC; Synthase; Genes; Polypeptides

Ethylene has been known since the beginning of the century to be used by plants as a signaling molecule for regulating a variety of developmental processes and stress responses, including seed germination, leaf and flower senescence, fruit ripening, cell elongation, nodulation and wound and pathogen responses. In the last few years, it has become evident that the diversity and specificity of the ethylene responses are reflected in the complexity of its biosynthesis and perception. The enzyme 1-aminocyclopropane-1-carboxylic(ACC) synthase converts S-adenosylmethionine to ACC, the precursor of ethylene, and methylthioadenosine. The specific aims of this proposal are to initiate and study the biochemical characterization of the Arabidopsis ACC polypeptides and characterize their expression patterns by RNA hybridization analysis or microarray technology.Ethylene is a unique plant signaling molecule. Its synthesis is regulated by a diverse array of inducers. Clearly characterization of the polypeptides involved in that synthesis will have both fundamental and practical importance.

自世纪初以来，已知乙烯被植物用作调节多种发育过程和胁迫响应的信号分子，所述发育过程和胁迫响应包括种子萌发、叶和花衰老、果实成熟、细胞伸长、成熟和创伤以及病原体响应。 在过去的几年中，它已经变得明显，乙烯反应的多样性和特异性反映在其生物合成和感知的复杂性。 1-氨基环丙烷-1-羧酸（ACC）合酶将S-腺苷甲硫氨酸转化为ACC（乙烯的前体）和甲硫腺苷。 本研究的具体目的是通过RNA杂交分析或微阵列技术，对拟南芥ACC多肽的生物化学特性进行研究，并对它们的表达模式进行表征。乙烯是植物特有的信号分子。 它的合成受多种诱导剂的调控。 清楚地表征参与该合成的多肽将具有基本和实际的重要性。