Enzymatically-based Sulfate Modification of Seaweed-Derived Polysaccharides

海藻多糖的酶法硫酸盐改性

• 批准号: 8960528
• 负责人: Debra Trantolo
• 金额: $ 5万
• 依托单位: Cambridge Scientific Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1990-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8960528&HistoricalAwards=false
• 关键词: Enzymatically; based; Sulfate; Modification; Seaweed

Marine biomass from seaweeds offers especially important commercial components for the food, pharmaceutical, and biotech industries. Components of marine biomass now finding commercial markets are alginates, agars, and carrageenans, all polysaccharides, i.e., polymeric carbohydrates or hydrocolloids. Unique to marine polysaccharides is the degree of sulfation; lower sulfate contents are generally considered high-value specialty items. In Phase I it is proposed to experimentally investigate the potential for identified sulfatase enzymes to cleave the sulfate groups on selected seaweed-derived polysaccharides with the objective of increasing their commercial value. To this end of a reduction in ester sulfation via sulfatase enzymes, comprehensive input/output assays will be performed, as well as an evaluation of rheological properties to identify improvements in gelling. In Phase II continuous flow process systems will be investigated using immobilized sulfatase enzymes with the objective of documenting and verifying percent conversions and reaction kinetics.

来自海藻的海洋生物量提供了特别重要的 用于食品、药品和 生物技术产业 海洋生物量的组成部分 寻找商业市场是藻酸盐，琼脂， 角叉菜胶，所有的多糖，即，聚合物 碳水化合物或水胶体。 海军特有的 多糖是硫酸化的程度;较低的硫酸盐 内容通常被认为是高价值的专业 项目. 在第一阶段，建议通过实验 研究鉴定硫酸酯酶的可能性 以裂解所选海藻衍生的 多糖，目的是增加它们的 商业价值。 为了减少酯 通过硫酸酯酶的硫酸化，综合 将进行输入/输出分析，以及 流变性能评价， 凝胶化的改进。 在第二阶段连续流中 过程系统将使用固定化 硫酸酯酶的目的是记录和 验证转化率和反应动力学。