Glucose Sensor Based on Ferrocene-Modified Poly (ethylene oxide) and Glucose Oxidase

基于二茂铁改性聚环氧乙烷和葡萄糖氧化酶的葡萄糖传感器

• 批准号: 8960426
• 负责人: Paul Hale
• 金额: $ 5万
• 依托单位: Moltech Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1990-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8960426&HistoricalAwards=false
• 关键词: Glucose; Sensor; Based; Ferrocene; Modified

This proposal describes the design and testing of a new class of amperometric glucose sensors in which electrical communication between the flavin redox centers of glucose oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a poly(ethylene oxide), or PEO, backbone. These polymeric relay systems combine several important features found in previous polymeric electron transfer relay systems: a) a flexible polymer backbone; b) the relays are attached to flexible side chains; c) PEO effectively complexes simple cationic species, such as potassium and sodium ions, which can give the polymer itself some cationic character and increase the interaction between the polymer and the negatively charged glucose oxidase molecules. The proposed research plan for Phase I concerns the development of this class of sensor through the following approach: a) synthesis and electrochemical characterization of several mediator (ferrocene) containing PEO materials; b) study of the interaction between flavin redox centers of glucose oxidase and the PEO-bound electron transfer relay system through investigation of these new flexible polymers in enzyme electrode configurations; c) covalent attachment of glucose oxidase to ferrocene-modified PEO in order to further increase the interaction between the enzyme and the mediators, to minimize competitive reoxidation of reduced glucose oxidase by dissolved oxygen, and to improve the long-term stability of the sensor.

该提案描述了一种新的 一类电流型葡萄糖传感器， 葡萄糖的黄素氧化还原中心之间的通讯 氧化酶和电极的连接是通过以下网络实现的： 与聚乙烯化学键合的供体-受体继电器 氧化物）或PEO主链。 这些聚合物中继系统 联合收割机的几个重要特征， 聚合物电子转移中继系统：a）柔性 B）所述继电器连接到柔性聚合物主链; 侧链; c）PEO有效地络合简单阳离子 离子，如钾和钠离子，可以给 聚合物本身具有一定的阳离子特性， 聚合物和带负电荷的 葡萄糖氧化酶分子。 拟议的研究计划 第一阶段涉及这类传感器的发展 （a）综合和 几种介体的电化学表征 B）含（二茂铁）的PEO材料的研究; 葡萄糖氧化酶黄素氧化还原中心间的相互作用 和PEO结合的电子转移中继系统， 这些新型柔性聚合物在酶中研究 电极配置; c）葡萄糖的共价连接 氧化酶到二茂铁修饰的PEO，以进一步 增加酶和蛋白质之间的相互作用， 介质，以尽量减少竞争性再氧化的减少 葡萄糖氧化酶溶解氧，并提高 传感器的长期稳定性。