Development of Modern Analytical Centrifugation Instruments and Methods

超离心能提供直接的定量信息 关于生物的纯度、大小、形状和相互作用， 大分子 现代分析的发展 用于大分子的超浓缩方法和自动化方法 用于分析和解释超声波数据， 对分子生物学的进步很重要。 处理的任务 包括：1）完成真实的开发- 时间，重力扫描沉积速度分析， 包括整合基于图形的数据解释 软件，2）开发分析方法， 分子之间的关联是异质的 相对于浮力质量，以及3）调整实时 干涉光学系统的XL-A超精密仪器。 的 最终目标是为科学界提供一个 现代化的全自动仪器和软件， 完整的流体动力学和热力学分析。

Ultracentrifugation can provide direct, quantitative information concerning the purity, size, shape and interactions of biological macromolecules. The development of a modern analytical ultracentrifuge methods for macromolecules and automated methods for the analysis and interpretation of ultracentrifugal data is important for advances in molecular biology. The tasks addressed by this proposal include: 1) completing the development of real- time, gravitational-sweep sedimentation velocity analysis, including the integration of graphics-based data interpretation software, 2) development of methods for the analysis of associations between molecules which are heterogeneous with respect to buoyant mass, and 3) adapting the real-time interference optical system to the XL-An ultracentrifuge. The ultimate goal is to provide the scientific community with a modern, fully-automated instrument and software capable of complete hydrodynamic and thermodynamic analyses.