Development of Modern Analytical Centrifugation Instruments and Methods

超离心可以提供有关生物大分子的纯度、大小、形状和相互作用的直接定量信息。现代分析大分子的超离心方法和超离心数据分析和解释的自动化方法的发展对分子生物学的进步是重要的。本建议处理的任务包括：1)完成实时重力扫描沉降速度分析的开发，包括基于图形的数据解释软件的集成；2)开发与浮力质量相关的非均质分子之间关联的分析方法；3)将实时干涉光学系统应用于XL-An超离心机。最终目标是为科学界提供能够完成流体动力学和热力学分析的现代化全自动仪器和软件。

Ultracentrifugation can provide direct, quantitative information concerning the purity, size, shape and interactions of biological macromolecules. The development of a modern analytical ultracentrifuge methods for macromolecules and automated methods for the analysis and interpretation of ultracentrifugal data is important for advances in molecular biology. The tasks addressed by this proposal include: 1) completing the development of real- time, gravitational-sweep sedimentation velocity analysis, including the integration of graphics-based data interpretation software, 2) development of methods for the analysis of associations between molecules which are heterogeneous with respect to buoyant mass, and 3) adapting the real-time interference optical system to the XL-An ultracentrifuge. The ultimate goal is to provide the scientific community with a modern, fully-automated instrument and software capable of complete hydrodynamic and thermodynamic analyses.