Innovative Technologies for Analytical Ultracentrifugation

分析超速离心的创新技术

• 批准号: 9876582
• 负责人: Thomas Laue
• 金额: $ 100万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9876582&HistoricalAwards=false
• 关键词: Innovative; Technologies; Analytical; Ultracentrifugation

AbstractThe specific interaction between molecules is at the core of all biological processes. As an instrument, the analytical ultracentrifuge has been a core technology for characterizing the strength, shape and size of molecular complexes. For many biological questions, there is no substitute method for analyzing molecular interactions. However, the existing analytical ultracentrifuge is limited in the range of questions it can address. New and improved optical systems described in this proposal will overcome many of these limitations, thus allowing the analysis of trace materials, improving the precision of the measurements 100-fold and extending the analysis of concentrated mixtures by a factor of 10. Specifically, fluorescence, schlieren and turbidity detectors will be added. Improvements will be made to the computer systems that operate these detectors, and improvements will be made to the computer program used for interpreting sedimentation data

分子间特定的相互作用是所有生物过程的核心。分析型超离心机作为一种仪器，已成为表征分子配合物强度、形状和大小的核心技术。对于许多生物学问题，没有分析分子相互作用的替代方法。然而，现有的分析式超离心机在它能解决的问题范围内是有限的。本提案中描述的新型和改进的光学系统将克服许多这些限制，从而允许分析痕量材料，将测量精度提高100倍，并将浓缩混合物的分析扩展到10倍。具体来说，荧光、纹影和浊度检测器将被添加。将改进操作这些探测器的计算机系统，并改进用于解释沉降数据的计算机程序