Innovative Technologies for Analytical Ultracentrifugation

摘要分子间的特定相互作用是所有生物过程的核心。作为一种仪器，分析超速离心机已成为表征分子络合物强度、形状和大小的核心技术。对于许多生物学问题，没有可替代的方法来分析分子相互作用。然而，现有的分析超速离心法仅限于它所能解决的问题范围。本建议中描述的新的和改进的光学系统将克服许多这些限制，从而允许分析痕量材料，将测量精度提高100倍，并将浓缩混合物的分析扩展到原来的10倍。具体地说，将增加荧光、纹影和浊度检测器。将改进操作这些探测器的计算机系统，并改进用于解释沉积数据的计算机程序

AbstractThe specific interaction between molecules is at the core of all biological processes. As an instrument, the analytical ultracentrifuge has been a core technology for characterizing the strength, shape and size of molecular complexes. For many biological questions, there is no substitute method for analyzing molecular interactions. However, the existing analytical ultracentrifuge is limited in the range of questions it can address. New and improved optical systems described in this proposal will overcome many of these limitations, thus allowing the analysis of trace materials, improving the precision of the measurements 100-fold and extending the analysis of concentrated mixtures by a factor of 10. Specifically, fluorescence, schlieren and turbidity detectors will be added. Improvements will be made to the computer systems that operate these detectors, and improvements will be made to the computer program used for interpreting sedimentation data