Collaborative Research: Dietary Deficiencies of Protein and Lipid and the Experimental Resolution of 'Food Limitation' in Freshwater Zooplankton

合作研究：淡水浮游动物蛋白质和脂质的膳食缺乏以及“食物限制”的实验解决

• 批准号: 9007640
• 负责人: Clyde Goulden
• 金额: $ 17.47万
• 依托单位: Academy of Natural Sciences Philadelphia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-15 至 1993-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9007640&HistoricalAwards=false
• 关键词: Collaborative; Research; Dietary; Deficiencies; Protein

Food limitation in herbivorous zooplankton (Daphnia, Diaptomus, also rotifers and protozoa) will be assayed using a recently developed technique--amendments of natural diets by additions of custom formulated microparticles containing lipid and fatty acids or proteins of various amino acid compositions. Concurrent analyses of diet--as (1) food items ingested, (2) biochemical composition of 30 um seston, (3) nutrient- limitations of ingestable algae, and (4) dietary deficiency effects on reproduction --will contrast spring ve summer conditions of nutrient--poor vs nutrient-rich lakes. Researchers focus field and laboratory experiments on potential compensation mechanisms such as diet breadth vs selectivity, excess feeding, omnivory and vertical migration as points of contrast between a generalist grazer (Daphnia) and a more selective feeder (Diaptomus), and test the idea that the "microbial loop" is effectively though differently utilized by both strategies.

食草性浮游动物（水蚤， Diaptomus，也包括轮虫和原生动物）将使用 最近开发的技术-通过 添加含有脂质的定制配方微粒 和各种氨基酸组成的脂肪酸或蛋白质。 饮食的同步分析--（1）摄入的食物，（2） 30 μ m悬浮物的生化组成; 藻类可摄取量的限制;（4）饮食不足 对繁殖的影响--将对比春季和夏季 贫营养湖泊与富营养湖泊的状况。 研究人员 电位补偿的现场和室内实验 如饮食宽度与选择性，过度喂养， 杂食性和垂直迁移之间的对比点， 多面手食草动物（水蚤）和更有选择性的喂食者 （Diaptomus），并测试“微生物循环”是 有效地，虽然不同地利用这两种策略。