Patch-Clamp Recording from Dissociated Hydra Cells

水螅是拥有神经系统的最原始的后生动物。 这种多细胞动物一直是 发育研究，但电生理学研究是 几乎不存在 由于一些技术原因， 应用传统的电生理技术， 水螅虫已经被证明是相当困难的， 成功 本研究将研究的性质， 应用膜片钳技术分离水螅神经元 夹紧。 这种微电极技术可以记录 细胞的离子通道太小， 传统的电生理学方法。 的效果 推测的神经递质对全细胞离子电流和单个 水螅神经和上皮细胞膜的通道活性 将被研究。 这项研究的结果将提供额外的 化学物质膜功能和生理学信息 水螅细胞中的传输。

Hydra is the most primitive metazoan possessing a nervous system. This multicellular animal has been a favorite model for developmental studies, yet electrophysiological studies are practically non-existent. For a number of technical reasons the application of traditional electrophysiological techniques using hydrozoans have proven to be quite difficult and overall not successful. This study will examine the properties of dissociated Hydra neurons by utilizing the technique of patch clamping. This microelectrode technique permits the recording of ion channels from cells too small that would not be possible with traditional electrophysiological methods. The effects of a putative neurotransmitter on whole cell ionic currents and single channels activity of hydra nerve and epithelial cell membranes will be studied. Results from this study will provide additional information on membrane function and physiology of chemical transmission in hydra cells.