Assembly of a Core Facility for Biological and Biochemical Research -- A Laboratory for Protein/Peptide Synthesis, Purification, and Characterization

生物生化研究核心设施组装——蛋白质/肽合成、纯化和表征实验室

• 批准号: 9011409
• 负责人: Richard Ikeda
• 金额: $ 21.7万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9011409&HistoricalAwards=false
• 关键词: Assembly; Core; Facility; Biological; Biochemical

The purpose of this application is to solicit support for the establishment of a basic core facility for Protein/Peptide Synthesis, Purification, and Characterization in the School of Chemistry and Biochemistry at the Georgia Institute of Technology. The facility is being established to provide routine access to the major pieces of basic biochemical/biological instrumentation. The PIs are requesting an amino acid analyzer, an autoclave, an ultracentrifuge, a high speed centrifuge, a cold room, a densitometer, a fermenter, a FPLC, a HPLC, a protein/peptide sequencer, a peptide synthesizer, and a scintillation counter to equip this core facility. The core facility for protein/peptide synthesis, purification, and characterization will be used primarily by five different research groups whose present access to modern pieces of biological and biochemical equipment is either extremely limited or nonexistant. The facility will greatly enhance our capability to investigate the effects of ultraviolet radiation on eye lens proteins, the molecular basis of function and regulation of T7 RNA polymerase, the effect of mutations on the folding pathway of ubiquitin, the design and synthesis of specific and effective protease inhibitors, and the crystal structures of pea lectin and human serum transferrin.

本申请的目的是为在乔治亚理工学院化学与生物化学学院建立一个用于蛋白质/肽合成、纯化和表征的基本核心设施寻求支持。该设施的建立是为了提供日常使用基本生化/生物仪器的主要部件。pi需要一个氨基酸分析仪，一个高压灭菌器，一个超离心机，一个高速离心机，一个冷室，一个密度计，一个发酵罐，一个FPLC，一个HPLC，一个蛋白质/肽测序仪，一个肽合成器，和一个闪烁计数器来装备这个核心设施。蛋白质/肽合成、纯化和表征的核心设施将主要由五个不同的研究小组使用，这些研究小组目前使用现代生物和生化设备的途径要么非常有限，要么根本不存在。该设施将极大地增强我们研究紫外线辐射对眼球晶体蛋白的影响、T7 RNA聚合酶的功能和调控的分子基础、突变对泛素折叠途径的影响、特异性和有效的蛋白酶抑制剂的设计和合成、豌豆凝集素和人血清转铁蛋白的晶体结构的能力。