Development of Tandem Mass Spectrometry for Analysis of Complex Biomolecules.

该奖项继续支持串联质谱活动 中大西洋质谱（MAMS）实验室 马里兰州巴尔的摩县大学。 参加的会议之一 采用质谱法（MS），串联MS提供了高质量的 高分子化合物的分辨率测量 蛋白质、核酸和其他生物聚合物的典型重量。 由该奖项赞助的努力将集中在改进技术上 用于蛋白质、糖蛋白和碳水化合物的分析， 串联仪器;各种合作研究项目 将开始或继续进行的培训活动， 博士后工作将继续进行。 质谱分析在生物医学中起着越来越重要的作用。 生物分子结构分析。 蛋白质信息 序列和结构，包括天然存在的修饰 蛋白质和多糖的类似信息 和其它支链聚合物， 其他技术的困难。 MAMS实验室包括 在UMBC和约翰霍普金斯大学的设施， 药 两者都是作为共享仪器资源建立的， 合作和服务项目。 由于大量的 购买和操作串联MS仪器的费用， 在大多数研究中， 机构职能体系 UMBC设施为研究界提供了 在UMBC和许多其他机构， 现代工业的许多领域都需要熟练的质谱分析技术 生物学研究

This award continues support of Tandem Mass Spectrometry activities at the Mid Atlantic Mass Spectrometry (MAMS) laboratory at the University of Maryland Baltimore County (UMBC). One of several approaches to mass spectrometry (MS), tandem MS provides high resolution measurements of compounds with the high molecular weights typical of proteins, nucleic acids and other biopolymers. Efforts sponsored by this award will focus on improving techniques for analysis of proteins, glycoproteins and carbohydrates using the tandem instrument; a variety of collaborative research projects will be initiated or continued, and training activities at the pre- and post doctoral level will continue. Mass spectrometry plays an increasingly important role in the structural analysis of biomolecules. Information about protein sequence and structure, including naturally occurring modifications of proteins, and similar kinds of information about polysaccharides and other branched chain polymers are obtained with great difficulty by other techniques. The MAMS laboratory includes facilities at UMBC and at The Johns Hopkins University School of Medicine. Both were established as shared instrument resources for collaborative and service projects. Because of the considerable expense of acquisition and operation of tandem MS instruments, this type of analysis is not routinely available at most research institutions. The UMBC facility provides the research community at UMBC and at numerous other institutions with access to the kind of skilled mass spectrometry needed in many areas of modern biological research.