权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Addition of Tandem Mass Spectrometry to the Middle Atlantic Mass Spectrometry Facility for the Analysis of Complex Biomolecules

在中大西洋质谱设施中添加串联质谱以分析复杂生物分子

基本信息

批准号：
8714238
负责人：
Catherine Fenselau
金额：
$ 128.67万
依托单位：
University of Maryland Baltimore County
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1987
资助国家：
美国
起止时间：
1987-09-15 至 1990-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=8714238&HistoricalAwards=false
关键词：
Addition Tandem Mass Spectrometry Middle

项目摘要

This is a Biological Facilities Center request for the purchase and developmental support of a tandem mass spectrometer, or MS/MS, to be used as a shared instrumental facility and as part of the Middle Atlantic Mass Spectrometry Facility. The instrument, to be located on the Baltimore County campus of the University of Maryland, will supplement existing capabilities available at the MAMS Facility at the Johns Hopkins University. Core and collaborative research by the principal investigators and their staff will include both instrument development and applications to the analysis of complex biomolecules. Instrument development will focus on improving the yield of fragment ion information for larger molecules using both collisions and laser photodissociation in the field.free region between MSl and MS2. Multiple collisions will be employed to induce fragmentation,neutralize molecular and fragment ions and equilibrate ion kinetic and internal energy. Fragment ions will then bereionized by chemical ionization and permit quadratic B scans in the second mass spectrometer at higher resolution than the B/Escans normally employed for high.energy collisions. MS/MS will be used to induce structural fragmentation, improve the sample ion signal relative to matrix ions, distinguish fragment ions formed from several molecular ion species, and separate components of microheterogeneous and other mixtures not readily separable by liquid chromatographic techniques. These advantages will be exploited for the structural analyses ofglycopeptides, lipopolysaccharides, chemically and post. translationally modified peptides, synthetic nucleotides, etc. in ongoing collaborative projects at the facility.

这是一份生物设施中心的申请，要求购买串联质谱仪或MS/MS，作为共享仪器设施和中大西洋质谱学设施的一部分。该仪器将设在马里兰大学巴尔的摩县校区，将补充约翰·霍普金斯大学MAMS设施现有的能力。主要研究人员及其工作人员的核心和协作研究将包括仪器开发和复杂生物分子分析的应用。仪器的开发将集中于提高更大分子碎片离子信息的产额，在MS1和MS2之间使用碰撞和激光光解离场。多重碰撞将被用来诱导碎裂，中和分子和碎片离子，平衡离子动能和内能。碎片离子将被化学电离电离，并允许在第二个质谱仪中以比通常用于高能碰撞的B/Escans更高的分辨率进行二次B扫描。MS/MS将用于诱导结构碎裂，改善相对于基质离子的样品离子信号，区分由几个分子离子物种形成的碎片离子，以及分离微均相和其他难以用液相色谱技术分离的混合物的组分。这些优势将被用于糖肽、脂多糖、化学和POST的结构分析。在该设施正在进行的合作项目中进行翻译修饰的多肽、合成核苷酸等。