Upgrade for a 500 mHz NMR Instrument

请求提供资金，以更新通用电气公司的GN-500核磁共振仪 带有附加探头的仪器，宽带去耦单元 (for多量子实验），一个高 分辨率倍增器（用于自旋锁实验），以及 改善仪器性能并促进数据处理的硬件 处理. 要求提供资金的一些项目 自该仪器早期安装以来，我一直被租借给UMBC 1988年，允许启动几个研究项目 介绍了 这一升级将允许继续结构 锌指-核酸复合物及完整的 HIV-1蛋白p7复合物的结构与构象 来自血型糖蛋白和细菌的碳水化合物 胶囊的立体化学和构象 丙二烯二肽电子等排体;底物内平衡;分子动力学 和产物在酵母糖苷酶的活性位点， 化学和酶促异构化的中间体 4-雄甾烯-3，17-二酮和葡糖苷酸异构体的结构 以及视黄酸盐、甲状腺素和谷胱甘肽结合物， 加压素

Funds are requested to upgrade a General Electric GN-500 NMR instrument with additional probes, a broad-band decoupling unit (for heteronuclear multiple quantum experiments), a high resolution decoupler (for spin-lock experiments), and the hardware to improve instrument performance and facilitates data processing. Some of the items for which funds are requested have been on loan to UMBC since the instrument was installed in early 1988, permitting initiation of several of the research programs described. This upgrade will permit continuation of structural studies of zinc finger--nucleic acid complexes and the intact HIV-1 protein p7; the structures and conformations of complex carbohydrates from blood group glycoproteins and bacteria capsules; the stereochemistry and conformation of novel allene dipeptide isosteres; the internal equilibrium of substrate and product in the active site of yeast glyoxalase, the intermediates in chemical and enzymatic isomerization of 4-androstene-3,17-dione, and structures of isomeric glucuronide and glutathione conjugates of retinoates, thyroxine and vasopressin.